Kozawa O, Takatsuki K, Kotake K, Yoneda M, Oiso Y, Saito H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
FEBS Lett. 1989 Jan 30;243(2):183-5. doi: 10.1016/0014-5793(89)80126-7.
In cloned osteoblast-like cells, MC3T3-E1, 12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C activating phorbol ester, and 1-oleoyl-2-acetylglycerol (OAG), a specific activator for protein kinase C, stimulated DNA synthesis in a dose-dependent manner. Both TPA and OAG acted synergistically with insulin-like growth factor I to stimulate DNA synthesis. TPA as well as OAG suppressed the increase in alkaline phosphatase activity of MC3T3-E1 cells induced by parathyroid hormone. These results suggest that protein kinase C is involved in the process which directs osteoblast-like cells toward proliferation.
