Ong J L, Villarreal D R, Cavin R, Ma K
University of Texas Health Science Center at San Antonio, Department of Restorative Dentistry, Division of Biomaterials, MSC 7890, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
J Mater Sci Mater Med. 2001 Jun;12(6):491-5. doi: 10.1023/a:1011259311032.
The clinical success of dental implants is governed by implant surfaces and bone cell responses that promote rapid osseointegration. The objective of this study was to evaluate the in vitro osteoblast cell response to heat treated and non-heat treated CaP coatings. In this study, the heat treated surfaces exhibited a poorly crystallized HA-type structure whereas the non-heat treated surface exhibited an amorphous structure. The heat treated CaP surfaces were observed to have a mean contact angle measurement of 57.95+/-0.95 degrees, whereas the non-heat treated CaP surfaces were observed to have a mean contact angle measurement of 44.6+/-0.3 degrees. From the in vitro cell culture study, the ATTC CRL 1486 human embryonic palatal mesenchyme (HEPM) cells displayed a similar protein production and hexosaminidase activity on the heat treated and non-heat treated CaP surfaces throughout the nine day experiment. However, the HEPM cells cultured on non-heat treated CaP surfaces were observed to have higher specific ALP activity after nine days' incubation compared to cells cultured on heat treated CaP surfaces. The higher specific ALP activity by cells on non-heat treated surfaces were suggested to be attributed to the lower degree of crystallinity and the lower contact angles observed in this study.
牙种植体的临床成功取决于种植体表面以及促进快速骨结合的骨细胞反应。本研究的目的是评估体外成骨细胞对热处理和未热处理的磷酸钙（CaP）涂层的反应。在本研究中，热处理表面呈现出结晶度较差的HA型结构，而未热处理表面呈现出无定形结构。观察到热处理的CaP表面的平均接触角测量值为57.95±0.95度，而未热处理的CaP表面的平均接触角测量值为44.6±0.3度。从体外细胞培养研究来看，在整个九天的实验过程中，美国典型培养物保藏中心（ATCC）CRL 1486人胚胎腭间充质（HEPM）细胞在热处理和未热处理的CaP表面上表现出相似的蛋白质产生和己糖胺酶活性。然而，与在热处理的CaP表面上培养的细胞相比，观察到在未热处理的CaP表面上培养的HEPM细胞在培养九天后具有更高的特异性碱性磷酸酶（ALP）活性。本研究表明，未热处理表面上的细胞具有较高的特异性ALP活性是由于其较低的结晶度和较低的接触角。
