Jiangsu Marine Fisheries Research Institute, Nantong, Jiangsu 226007, China; Natural History Museum, Department of Life Sciences, Cromwell Road, London SW7 5BD, United Kingdom.
Jiangsu Marine Fisheries Research Institute, Nantong, Jiangsu 226007, China; Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China.
Mol Phylogenet Evol. 2018 Mar;120:94-102. doi: 10.1016/j.ympev.2017.11.009. Epub 2017 Nov 24.
A molecular taxonomic study was undertaken for the first time of the bladed Bangiales of the mainland coast of China (Northwest Pacific) based on sequence data of 201 plastid rbcL and 148 nuclear 18S sequences of historical and contemporary specimens. The results revealed that only one genus of bladed Bangiales, Pyropia, was present along Chinese coast. Species delimitation was determined using two empirical methods: the Automatic Barcode Gap Discovery (ABGD) and General Mixed Yule Coalescence (GMYC) coupled with detection of monophyly in tree reconstruction. At least fourteen species of Pyropia were recovered. Six species were confirmed that had been recorded previously based on morphology (Py. suborbiculata, Py. yezoensis, Py. haitanensis, Py. katadae, Py. tenera and Py. acanthophora), three species were recorded from China for the first time (Py. kinositae, Py. pseudolinearis and Py. tanegashimensis), and five cryptic species that did not match any molecular sequences were also discovered. The phylogeny of the concatenated rbcL and 18S dataset resolved three singletons and four clades. Each clades has a strong trend towards occupying a biogeographic region, but they are not confined to them. A transoceanic and antitropical pattern of distribution was found for Pyropia at both the subgeneric and species level. This together with high biodiversity (ca. 30% of all known Pyropia species) indicates that the Northwest Pacific might act as a centre of origin for modern distribution of Pyropia since the early Cenozoic.
首次对中国（西北太平洋）大陆沿海的 blade Bangiales 进行了分子分类学研究，研究基于历史和当代标本的 201 个质体 rbcL 和 148 个核 18S 序列的数据。研究结果表明，中国沿海只有一个 blade Bangiales 属，Pyropia。使用两种经验方法（自动条形码间隙发现（ABGD）和广义混合尤尔 coalescence（GMYC））结合树重建中的单系检测来确定物种界限。至少恢复了 14 种 Pyropia。确认了六种以前基于形态学记录的物种（Py. suborbiculata、Py. yezoensis、Py. haitanensis、Py. katadae、Py. tenera 和 Py. acanthophora），三种在中国首次记录的物种（Py. kinositae、Py. pseudolinearis 和 Py. tanegashimensis），以及五种未与任何分子序列匹配的隐种。联合 rbcL 和 18S 数据集的系统发育解析了三个单体和四个分支。每个分支都有强烈的趋势占据一个生物地理区域，但它们并不局限于这些区域。在亚属和种水平上，Pyropia 都表现出跨洋和反热带分布模式。这与高生物多样性（约占所有已知 Pyropia 物种的 30%）一起表明，西北太平洋可能自新生代早期以来就是 Pyropia 现代分布的起源中心。
