Chen W X, Alley M R, Manktelow B W
Department of Veterinary Pathology and Public Health, Massey University, New Zealand.
J Comp Pathol. 1989 Jan;100(1):77-89. doi: 10.1016/0021-9975(89)90092-3.
The intranasal inoculation of mice with a suspension of ovine isolates of Bordetella parapertussis killed about 11 per cent of animals (four out of 34) between 2 to 3 days post-inoculation and produced a subacute bronchopneumonia morphologically similar to early lesions of naturally occurring ovine chronic non-progressive pneumonia. Pulmonary lesions characterized by alveolar septal congestion and oedema, focal alveolar haemorrhage and intraalveolar and septal infiltration by neutrophils and macrophages were seen in the early stages. Bronchioles often contained an exudate of macrophages, neutrophils and fibrin and later hyperplasia of perivascular and peribronchiolar lymphoid tissue and fibrosis of the interalveolar septa were evident. Electron microscopy revealed extensive degenerative changes in the alveolar epithelium and alveolar macrophages. Pure cultures of B. parapertussis were consistently recovered from infected lungs from 12 h after inoculation to pid 6. Intact organisms and organisms showing varying degrees of degeneration were found free in alveolar spaces or phagocytosed by alveolar macrophages.
用副百日咳博德特氏菌羊分离株悬液经鼻接种小鼠后，在接种后2至3天内约11%的动物（34只中有4只）死亡，并产生了一种亚急性支气管肺炎，其形态学与自然发生的羊慢性非进行性肺炎的早期病变相似。早期可见以肺泡间隔充血和水肿、局灶性肺泡出血以及中性粒细胞和巨噬细胞在肺泡内和间隔浸润为特征的肺部病变。细支气管常含有巨噬细胞、中性粒细胞和纤维蛋白的渗出物，随后血管周围和细支气管周围淋巴组织增生以及肺泡间隔纤维化明显。电子显微镜显示肺泡上皮和肺泡巨噬细胞有广泛的退行性变化。从接种后12小时到感染后第6天，始终能从感染的肺中分离出副百日咳博德特氏菌的纯培养物。在肺泡腔中发现完整的细菌以及显示不同程度退化的细菌，或被肺泡巨噬细胞吞噬。
