溶血巴斯德氏菌与副百日咳博德特氏菌之间的相互作用对肺巨噬细胞体外吞噬作用的影响。

Effects of interactions between Pasteurella haemolytica and Bordetella parapertussis on in vitro phagocytosis by lung macrophages.

作者信息

Hodgson J C, Brennand S E, Porter J F

机构信息

Moredun Research Institute, Edinburgh, U.K.

出版信息

Biologicals. 1996 Dec;24(4):325-8. doi: 10.1006/biol.1996.0045.

DOI:10.1006/biol.1996.0045

PMID:9088547

Abstract

The aim of the work was to determine the effect of exposing ovine bronchoalveolar macrophages (BAM) in vivo to Pasteurella haemolytica and/or Bordetella parapertussis on the subsequent uptake and killing of P. haemolytica by these cells in vitro. Exposure in vivo to P. haemolytica did not affect the uptake of P. haemolytica by BAM in vitro but reduced (P < 0.05) the intracellular killing of bacteria. Exposure in vivo to B. parapertussis had no significant effect on either the uptake or killing of P. haemolytica in vitro. However, sequential exposure in vivo to B. parapertussis and P. haemolytica reduced both the ingestion (P < 0.05) and killing (P < 0.001) of P. haemolytica in vitro. These results indicate that exposure to P. haemolytica compromised the bacterial killing mechanisms of BAM and that synergy between B. parapertussis and P. haemolytica reduced the ability of BAM to ingest bacteria.

摘要

这项工作的目的是确定体内将绵羊支气管肺泡巨噬细胞（BAM）暴露于溶血巴斯德氏菌和/或副百日咳博德特氏菌对这些细胞随后在体外摄取和杀灭溶血巴斯德氏菌的影响。体内暴露于溶血巴斯德氏菌对体外BAM摄取溶血巴斯德氏菌没有影响，但降低了（P<0.05）细胞内细菌的杀灭能力。体内暴露于副百日咳博德特氏菌对体外溶血巴斯德氏菌的摄取或杀灭均无显著影响。然而，体内先后暴露于副百日咳博德特氏菌和溶血巴斯德氏菌会降低体外溶血巴斯德氏菌的摄取（P<0.05）和杀灭能力（P<0.001）。这些结果表明，暴露于溶血巴斯德氏菌会损害BAM的细菌杀灭机制，并且副百日咳博德特氏菌和溶血巴斯德氏菌之间的协同作用会降低BAM摄取细菌的能力。