Chen W, Alley M R, Manktelow B W
Department of Veterinary Pathology, Massey University, Palmerston North, New Zealand.
Res Vet Sci. 1990 Jan;48(1):18-22.
A single dose of culture fluid of Bordetella parapertussis freed from cells (CFCF) given intranasally to four-week-old mice free from intercurrent respiratory disease produced a subacute bronchopneumonia, which was similar to that induced by whole cells of ovine isolates of B parapertussis, except that the lesions were less severe and less extensive. From eight hours to 17 days after inoculation, the mice exhibited marked infiltration of neutrophils and macrophages into the alveolar septa, bronchiolar and alveolar spaces, and hyperplasia of peribronchiolar and perivascular lymphoid tissue. Electron microscopy showed damage to ciliated cells, type 1 pneumocytes and alveolar macrophages. These results suggest that extracellular toxic substance(s) produced by ovine isolates of B parapertussis might be involved in the initiation and development of lesions in ovine chronic non-progressive pneumonia.
给四周龄且无并发呼吸道疾病的小鼠经鼻内接种单剂量无细胞的副百日咳博德特氏菌培养液（CFCF），可引发亚急性支气管肺炎，该肺炎与由副百日咳博德特氏菌绵羊分离株全细胞诱导的肺炎相似，只是病变程度较轻且范围较小。接种后8小时至17天，小鼠肺泡间隔、细支气管和肺泡腔内出现明显的中性粒细胞和巨噬细胞浸润，细支气管周围和血管周围淋巴组织增生。电子显微镜检查显示纤毛细胞、Ⅰ型肺泡上皮细胞和肺泡巨噬细胞受损。这些结果表明副百日咳博德特氏菌绵羊分离株产生的细胞外毒性物质可能参与了绵羊慢性非进行性肺炎病变的起始和发展。
