Porter J F, Mason C S, Krueger N, Connor K, Donachie W
Moredun Research Institute, Edinburgh, UK.
Vet Microbiol. 1995 Oct;46(4):393-400. doi: 10.1016/0378-1135(95)00045-c.
Initial intranasal inoculation of four to eight-week-old Swiss White mice with 7.5 x 10(6) colony forming units (cfu) of ovine B. parapertussis followed 30 min, three or five days, by intranasal inoculation with 1.4 x 10(5) cfu of Pasteurella haemolytica A2 resulted in a more severe infection pattern than when either agent was administered alone. Histopathological examination showed that inoculation with B. parapertussis alone caused a bronchopneumonia the severity of which was dependant upon the infecting dose. Bacteria were recovered up to 10 days after inoculation. P. haemolytica alone had no apparant pathogenic effect and was cleared from the lungs within 24 h. When both agents were given in combination the lesions were most severe when P. haemolytica was administered three days after B. parapertussis infection. These findings suggest that B. parapertussis predisposes mice to subsequent infection with P. haemolytica and that the timing of the P. haemolytica administration influences the severity of the lung lesions.
用7.5×10⁶集落形成单位（cfu）的羊副百日咳博德特氏菌对4至8周龄的瑞士小白鼠进行初次鼻内接种，30分钟后、3天或5天后，再用1.4×10⁵cfu的溶血巴斯德氏菌A2进行鼻内接种，与单独施用任何一种病原体相比，会导致更严重的感染模式。组织病理学检查表明，单独接种副百日咳博德特氏菌会引起支气管肺炎，其严重程度取决于感染剂量。接种后10天内均可分离到细菌。单独的溶血巴斯德氏菌没有明显的致病作用，并且在24小时内从肺部清除。当两种病原体联合使用时，在副百日咳博德特氏菌感染3天后施用溶血巴斯德氏菌时，病变最为严重。这些发现表明，副百日咳博德特氏菌使小鼠易患随后的溶血巴斯德氏菌感染，并且溶血巴斯德氏菌的施用时间会影响肺部病变的严重程度。
