Discovery Sciences, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Gothenburg, Sweden.
Department of Biology and Bioengineering, Chalmers University of Technology, Gothenburg, Sweden.
Stem Cell Rev Rep. 2018 Apr;14(2):177-188. doi: 10.1007/s12015-017-9783-8.
There is a need for physiologically relevant assay platforms to provide functionally relevant models of diabetes, to accelerate the discovery of new treatment options and boost developments in drug discovery. In this review, we compare several 3D-strategies that have been used to increase the functional relevance of ex vivo human primary pancreatic islets and developments into the generation of stem cell derived pancreatic beta-cells (β-cells). Special attention will be given to recent approaches combining the use of extracellular matrix (ECM) scaffolds with pancreatic molecular memory, which can be used to improve yield and functionality of in vitro stem cell-derived pancreatic models. The ultimate goal is to develop scalable cell-based platforms for diabetes research and drug screening. This article will critically assess key aspects related to in vitro pancreatic 3D-ECM models and highlight the most promising approaches for future research.
需要有生理相关的检测平台来提供更具功能相关性的糖尿病模型，以加速新的治疗方案的发现并推动药物研发的进展。在这篇综述中，我们比较了几种 3D 策略，这些策略被用于提高体外人原代胰腺胰岛的功能相关性，并发展为干细胞衍生的胰腺β细胞（β细胞）。特别关注的是最近将细胞外基质（ECM）支架与胰腺分子记忆结合使用的方法，这可用于提高体外干细胞衍生的胰腺模型的产量和功能。最终目标是开发用于糖尿病研究和药物筛选的可扩展的基于细胞的平台。本文将批判性地评估与体外胰腺 3D-ECM 模型相关的关键方面，并强调未来研究中最有前途的方法。
