Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, ul. Oczapowskiego 13, 10-719 Olsztyn, Poland.
Poult Sci. 2018 Feb 1;97(2):470-476. doi: 10.3382/ps/pex341.
The progressive decrease in the efficiency of synthetic drugs has prompted research into phytogenic feed additives with potentially immunomodulatory and anti-infective properties. Complex diseases with a mixed etiology, including viral, pose a growing problem in domestic pigeons. The aim of this study was to determine the effectiveness of various doses of aloe vera and licorice extracts on the course of experimental PPMV-1 infection in pigeons. The experiment was performed on pigeons divided into 5 groups, including one control group and 4 experimental groups, which were orally administered aloe vera or licorice extracts at 300 or 500 mg/kg BW for 7 d after experimental inoculation with PPMV-1. On d 4, 7, and 14 after inoculation, cloacal swabs and samples of organs were collected from 4 birds in each group. The samples were analyzed to determine the copy number of PPMV-1 RNA by TaqMan qPCR. The results indicate that licorice and aloe vera extracts inhibited PPMV-1 replication by decreasing viral RNA copy numbers in the examined organs. The most inhibitory effect was observed in pigeons receiving aloe vera extract at 300 mg/kg BW, for which PPMV-1 RNA copy numbers were approximately 7-fold lower (brain), 9-fold lower (kidneys), and 14-fold lower (liver) than in the control group. The results of this study point to the potentially antiviral effects of aloe vera and licorice extracts in pigeons infected with PPMV-1. To the best of our knowledge, this is the first study to investigate the antiviral properties of aloe vera and licorice extracts in domestic pigeons.
合成药物功效逐渐降低，促使人们开始研究具有潜在免疫调节和抗感染特性的植物源性饲料添加剂。具有混合病因的复杂疾病，包括病毒，对家鸽造成越来越大的威胁。本研究旨在确定不同剂量的芦荟和甘草提取物对鸽子实验性 PPMV-1 感染病程的影响。实验在家鸽中进行，将家鸽分为 5 组，包括一组对照组和 4 组实验组，实验组在实验接种 PPMV-1 后 7 天内每天经口给予 300 或 500mg/kg BW 的芦荟或甘草提取物。接种后第 4、7 和 14 天，每组采集 4 只鸽子的泄殖腔拭子和器官样本。通过 TaqMan qPCR 分析样本以确定 PPMV-1 RNA 的拷贝数。结果表明，甘草和芦荟提取物通过降低检测器官中的病毒 RNA 拷贝数来抑制 PPMV-1 的复制。在接受 300mg/kg BW 芦荟提取物的鸽子中观察到最显著的抑制效果，其 PPMV-1 RNA 拷贝数比对照组低约 7 倍（大脑）、9 倍（肾脏）和 14 倍（肝脏）。本研究结果表明，芦荟和甘草提取物在家鸽感染 PPMV-1 时具有潜在的抗病毒作用。据我们所知，这是首次研究芦荟和甘草提取物在家鸽中的抗病毒特性。
