Zheng Shumei, Wang Xiaowei, Hu Huiqiong, Xia Yongbo, Diao Xiaoyuan, Qiu Wenjing, Xue Chunyi, Cao Yongchang, Xu Zhichao
State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China.
Front Vet Sci. 2022 Aug 18;9:978453. doi: 10.3389/fvets.2022.978453. eCollection 2022.
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an emerging swine enteropathogenic coronavirus that causes severe diarrhea in neonatal piglets, leading to serious economic losses to the pig industries. At present, there are no effective control measures for SADS, making an urgent need to exploit effective antiviral therapies. Here, we confirmed that Aloe extract (Ae) can strongly inhibit SADS-CoV in Vero and IPI-FX cells . Furthermore, we detected that Emodin from Ae had anti-SADS-CoV activity in cells but did not impair SADS-CoV infectivity directly. The time-of-addition assay showed that Emodin inhibits SADS-CoV infection at the whole stages of the viral replication cycle. Notably, we found that Emodin can significantly reduce virus particles attaching to the cell surface and induce TLR3 ( < 0.001), IFN-λ3 ( < 0.01), and ISG15 ( < 0.01) expressions in IPI-FX cells, indicating that the anti-SADS-CoV activity of Emodin might be due to blocking viral attachment and the activation of TLR3-IFN-λ3-ISG15 signaling axis. These results suggest that Emodin has the potential value for the development of anti-SADS-CoV drugs.
猪急性腹泻综合征冠状病毒（SADS-CoV）是一种新出现的猪肠道致病性冠状病毒，可导致新生仔猪严重腹泻，给养猪业造成严重经济损失。目前，针对SADS尚无有效的控制措施，因此迫切需要开发有效的抗病毒疗法。在此，我们证实芦荟提取物（Ae）能在Vero细胞和IPI-FX细胞中强烈抑制SADS-CoV。此外，我们检测到Ae中的大黄素在细胞中具有抗SADS-CoV活性，但不会直接损害SADS-CoV的感染性。添加时间试验表明，大黄素在病毒复制周期的整个阶段均抑制SADS-CoV感染。值得注意的是，我们发现大黄素可显著减少附着在细胞表面的病毒颗粒，并诱导IPI-FX细胞中TLR3（<0.001）、IFN-λ3（<0.01）和ISG15（<0.01）的表达，这表明大黄素的抗SADS-CoV活性可能是由于阻断病毒附着以及激活TLR3-IFN-λ3-ISG15信号轴所致。这些结果表明，大黄素在抗SADS-CoV药物开发方面具有潜在价值。
