Kominato Y, Kumada K, Yamazaki K, Misawa S
Department of Legal Medicine, University of Tsukuba, Ibaraki-ken, Japan.
J Forensic Sci. 1989 Jan;34(1):207-17.
To estimate postmortem interval (PMI), spontaneous cleavage of the third component of complement (C3) was studied in aged blood and cadaveric blood by crossed immunoelectrophoresis. Using the kinetics of C3 cleavage in vitro described as dC/dt = -kC, where C is the concentration of native C3 at time t and k is a first-order rate constant, Arrhenius' equation, and another equation which assumes a linear drop of body temperature after death, the percentages of C3 cleavage were calculated. There was a significant positive correlation between the calculated percentages and the measured percentages of up to 10% in cadaveric blood. We found that the comparison between the calculated percentage of C3 cleavage for each optional postmortem interval and the measured percentage of up to 10% in cadaveric blood leads to the estimation of PMI. This approach is one step towards the development of an accurate method for determining PMI based on C3 cleavage, that is, on a first-order reaction.
为了估计死后间隔时间（PMI），通过交叉免疫电泳研究了衰老血液和尸体血液中补体第三成分（C3）的自发裂解。利用体外C3裂解动力学，其描述为dC/dt = -kC，其中C是时间t时天然C3的浓度，k是一级速率常数，根据阿伦尼乌斯方程以及另一个假设死后体温呈线性下降的方程，计算了C3裂解的百分比。在尸体血液中，计算出的百分比与测量出的百分比之间存在显著正相关，最高可达10%。我们发现，将每个可选死后间隔时间的C3裂解计算百分比与尸体血液中测量出的最高10%的百分比进行比较，可得出PMI的估计值。这种方法是朝着基于C3裂解（即基于一级反应）开发一种准确测定PMI的方法迈出的一步。
