Kominato Y, Harada S, Yamazaki K, Misawa S
Department of Legal Medicine, University of Tsukuba, Japan.
J Forensic Sci. 1988 Mar;33(2):404-9.
To estimate postmortem interval (PMI), the spontaneous conversion of the native third component of complement (C3) to its derived fragments in whole blood was studied by crossed immunoelectrophoresis. C3 cleavages in vitro at different temperatures showed that the incubation of whole blood at a higher temperature led to a faster conversion of beta 1C (native C3) to beta 1A (C3c). In cadaveric blood, we found a significant positive correlation between percentage of C3 cleavage and PMI. From these results, it is possible to estimate PMI from the ratios of C3 cleavage.
为了估计死后间隔时间（PMI），通过交叉免疫电泳研究了全血中补体天然第三成分（C3）向其衍生片段的自发转化。在不同温度下体外C3裂解表明，全血在较高温度下孵育会导致β1C（天然C3）更快地转化为β1A（C3c）。在尸体血液中，我们发现C3裂解百分比与PMI之间存在显著正相关。根据这些结果，可以从C3裂解比率估计PMI。
