State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Pestic Biochem Physiol. 2017 Nov;143:191-198. doi: 10.1016/j.pestbp.2017.07.007. Epub 2017 Jul 29.
The antifungal properties and the induction of resistance by ε-poly-l-lysine (ε-PL) were examined to reveal its potential in protecting tomato plants against Botrytis cinerea. As presented herein, ε-PL at 1200mg/L was found to have optimal in vitro antifungal activities, achieving an inhibition rate of 94.96%. In first-year field tests, ε-PL (1200mg/L) had a control effect of up to 79.07% against tomato grey mould. Similar results were obtained in the second year. In greenhouse experiments, ε-PL was observed to effectively reduce leaf infection, with an observed control rate at 89.22%. To define the molecular-genetic mechanisms, we compared the gene expression under four different conditions: sterile water sprayed plants (Control), Botrytis-infected plants (Inf), ε-PL-treated plants (ε-PL) and ε-PL-treated+infected plants (ε-PL+Inf). Quantitative PCR analysis at 36h after inoculation revealed that ε-PL+Inf plants exhibited significant expression and priming of several key Botrytis-induced genes in tomato. The results indicate that ε-PL promoted plant capacity of tomato to activate defense mechanisms upon pathogen attack. In total, these findings revealed that ε-PL should be an excellent biocontrol agent candidate that combined direct antifungal activity against B. cinerea and plant resistance capacity.
ε-聚赖氨酸（ε-PL）的抗真菌特性和诱导抗性的能力被研究，以揭示其在保护番茄植物免受灰霉病侵害方面的潜力。如本文所述，在体外试验中，发现 1200mg/L 的ε-PL 具有最佳的抗真菌活性，抑制率达到 94.96%。在第一年的田间试验中，ε-PL（1200mg/L）对番茄灰霉病的防治效果高达 79.07%。第二年也得到了类似的结果。在温室试验中，观察到ε-PL 能有效降低叶片感染，控制率为 89.22%。为了明确分子遗传机制，我们比较了四种不同条件下的基因表达：无菌水喷雾处理的植物（对照）、感染灰霉病的植物（感染）、ε-PL 处理的植物（ε-PL）和 ε-PL 处理+感染的植物（ε-PL+感染）。接种后 36 小时的定量 PCR 分析表明，ε-PL+感染的植物显著表达和启动了番茄中几个关键的灰霉病诱导基因。这些结果表明，ε-PL 促进了番茄植物在受到病原体攻击时激活防御机制的能力。总的来说，这些发现表明 ε-PL 应该是一种优秀的生物防治剂候选物，它结合了对 B. cinerea 的直接抗真菌活性和植物抗性能力。
