Biological Sciences Research Group, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
Institute for Bioengineering and Biosciences, Biological Sciences Research Group, Instituto Superior Técnico, Lisbon, Portugal.
Front Cell Infect Microbiol. 2017 Nov 14;7:473. doi: 10.3389/fcimb.2017.00473. eCollection 2017.
Persistence and virulence of infections are multifactorial phenomena, whose understanding is crucial to design more suitable therapeutic strategies. In this study, the putative multidrug transporter CgDtr1, encoded by ORF , is identified as a determinant of virulence in the infection model . deletion is shown to decrease the ability to kill larvae by decreasing ability to proliferate in hemolymph, and to tolerate the action of hemocytes. The possible role of CgDtr1 in the resistance to several stress factors that underlie death induced by phagocytosis was assessed. was found to confer resistance to oxidative and acetic acid stress. Consistently, CgDtr1 was found to be a plasma membrane acetic acid exporter, relieving the stress induced upon cells within hemocytes, and thus enabling increased proliferation and virulence against larvae.
感染的持续性和毒力是多因素现象，了解其机制对于设计更合适的治疗策略至关重要。在本研究中，假定的多药转运蛋白 CgDtr1，由 ORF 编码，被鉴定为感染模型中 毒力的决定因素。 缺失被证明会降低在血淋巴中增殖的能力，从而降低杀死 幼虫的能力，并耐受血细胞的作用。评估了 CgDtr1 在抵抗几种应激因素中的可能作用，这些应激因素是吞噬作用诱导死亡的基础。 被发现赋予对氧化和乙酸胁迫的抗性。一致地，CgDtr1 被发现是质膜乙酸外排体，减轻血细胞内 细胞受到的应激，从而使增殖和对 幼虫的毒力增加。
