Boynton Felicia D Duke, Ericsson Aaron C, Uchihashi Mayu, Dunbar Misha L, Wilkinson J Erby
Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA.
Research Animal Resources, University of Minnesota, Minneapolis, MN, USA.
BMC Res Notes. 2017 Nov 29;10(1):644. doi: 10.1186/s13104-017-2960-7.
This study aims to demonstrate the effect of oral doxycycline on fecal microbiota of mice. Doxycycline is a common effector for control of gene expression using the tet-inducible system in transgenic mice. The effect of oral doxycycline on murine gut microbiota has not been reported. We evaluated the effect of doxycycline treatment by sequencing the V4 hypervariable region of the 16S rRNA gene from fecal samples collected during a 4 week course of treatment at a dose of 2 mg/ml in the drinking water.
The fecal microbiota of treated animals were distinct from control animals; the decreased richness and diversity were characterized primarily by Bacteroides sp. enrichment. These effects persisted when the treatment was temporarily discontinued for 1 week. These data suggest that doxycycline treatment can induce significant dysbiosis, and its effects should be considered when used in animal models that are or maybe sensitive to perturbation of the gut microbiota.
本研究旨在证明口服强力霉素对小鼠粪便微生物群的影响。强力霉素是转基因小鼠中使用四环素诱导系统控制基因表达的常用效应物。口服强力霉素对小鼠肠道微生物群的影响尚未见报道。我们通过对在为期4周的治疗过程中收集的粪便样本中的16S rRNA基因的V4高变区进行测序，评估了以2mg/ml的剂量添加到饮用水中的强力霉素治疗的效果。
治疗组动物的粪便微生物群与对照组动物不同；丰富度和多样性的降低主要表现为拟杆菌属富集。当治疗暂时中断1周时，这些影响仍然存在。这些数据表明，强力霉素治疗可导致显著的生态失调，在使用对肠道微生物群扰动敏感或可能敏感的动物模型时，应考虑其影响。
