Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, P. R. China.
Sci Rep. 2017 Apr 20;7:46551. doi: 10.1038/srep46551.
Accumulating evidence suggests that the gut microbiota dysbiosis and their host metabolic phenotype alteration is an important factor in human disease development. A traditional Chinese herbal formula, Chaihu-Shu-Gan-San (CSGS), has been effectively used in the treatment of various gastrointestinal (GI) disorders. The present study was carried out to investigate whether CSGS modulates the host metabolic phenotype under the condition of gut microbiota dysbiosis. The metabonomics studies of biochemical changes in urine and feces of antibiotic-induced gut microbiota dysbiosis rats after treatment with CSGS were performed using UPLC-Q-TOF/MS. Partial least squares-discriminate analysis (PLS-DA) indicated that the CSGS treatment reduced the metabolic phenotype perturbation induced by antibiotic. In addition, there was a strong correlation between gut microbiota genera and urinary and fecal metabolites. Moreover, the correlation analysis and the metabolic pathway analysis (MetPA) identified that three key metabolic pathways including glycine, serine and threonine metabolism, nicotinate and nicotinamide metabolism, and bile acid metabolism were the most relevant pathways involved in antibiotic-induced gut microbiota dysbiosis. These findings provided a comprehensive understanding of the protective effects of CSGS on the host metabolic phenotype of the gut microbiota dysbiosis rats, and further as a new source for drug leads in gut microbiota-targeted disease management.
越来越多的证据表明，肠道微生物群落失调及其宿主代谢表型改变是人类疾病发展的一个重要因素。一种传统的中草药配方，柴胡疏肝散（CSGS），已被有效地用于治疗各种胃肠道（GI）疾病。本研究旨在探讨 CSGS 是否在肠道微生物群落失调的情况下调节宿主代谢表型。采用 UPLC-Q-TOF/MS 对 CSGS 治疗抗生素诱导的肠道微生物群落失调大鼠尿液和粪便中生化变化进行代谢组学研究。偏最小二乘判别分析（PLS-DA）表明，CSGS 治疗减轻了抗生素引起的代谢表型紊乱。此外，肠道微生物属与尿和粪便代谢物之间存在很强的相关性。此外，相关性分析和代谢途径分析（MetPA）确定了三个关键代谢途径，包括甘氨酸、丝氨酸和苏氨酸代谢、烟酸和烟酰胺代谢以及胆汁酸代谢，是与抗生素诱导的肠道微生物群落失调最相关的途径。这些发现为 CSGS 对肠道微生物群落失调大鼠宿主代谢表型的保护作用提供了全面的认识，并进一步为肠道微生物靶向疾病管理中的药物先导提供了新的来源。
