Qi Ji, He Chao, Elson Daniel S
Hamlyn Centre for Robotic Surgery, Institute of Global Health Innovation, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
Department of Surgery and Cancer, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
Biomed Opt Express. 2017 Oct 10;8(11):4933-4946. doi: 10.1364/BOE.8.004933. eCollection 2017 Nov 1.
Tissue polarimetric imaging measures Mueller matrices of tissues or Stokes vectors of the emergent light from tissues (normally using incidence with a fixed polarization state) over a field of view, and has demonstrated utility in a number of surgical and diagnostic applications. Here we introduce a compact complete Stokes polarimetric imager that can work for multiple wavelength bands with a frame-rate suitable for real-time applications. The imager was validated with standard polarizing components, and then employed as a polarization state analyzer of a Mueller imaging polarimeter and a standalone Stokes imaging polarimeter respectively to image the process of dehydration of bovine tendon tissue. The results obtained in this work suggested that the polarization properties of the samples rich of collagen fibres can change with the degree of dehydration, and therefore, dehydration of the samples prepared for polarimetric imaging (e.g. polarimetric microscopy) should be carefully controlled.
组织偏振成像可测量组织的穆勒矩阵或组织出射光的斯托克斯矢量(通常使用固定偏振态的入射光),覆盖一个视场,并已在许多外科手术和诊断应用中展现出实用性。在此,我们介绍一种紧凑的完整斯托克斯偏振成像仪,它可在多个波长波段工作,帧率适合实时应用。该成像仪通过标准偏振组件进行了验证,然后分别用作穆勒成像偏振计和独立斯托克斯成像偏振计的偏振态分析仪,对牛肌腱组织的脱水过程进行成像。这项工作获得的结果表明,富含胶原纤维的样品的偏振特性会随脱水程度而变化,因此,为偏振成像(如偏振显微镜)制备的样品的脱水过程应仔细控制。