Dong Yang, Qi Ji, He Honghui, He Chao, Liu Shaoxiong, Wu Jian, Elson Daniel S, Ma Hui
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Department of Biomedical Engineering, Tsinghua University, Beijing 100084, China.
Biomed Opt Express. 2017 Jul 13;8(8):3643-3655. doi: 10.1364/BOE.8.003643. eCollection 2017 Aug 1.
Polarization imaging has been recognized as a potentially powerful technique for probing the microstructural information and optical properties of complex biological specimens. Recently, we have reported a Mueller matrix microscope by adding the polarization state generator and analyzer (PSG and PSA) to a commercial transmission-light microscope, and applied it to differentiate human liver and cervical cancerous tissues with fibrosis. In this paper, we apply the Mueller matrix microscope for quantitative detection of human breast ductal carcinoma samples at different stages. The Mueller matrix polar decomposition and transformation parameters of the breast ductal tissues in different regions and at different stages are calculated and analyzed. For more quantitative comparisons, several widely-used image texture feature parameters are also calculated to characterize the difference in the polarimetric images. The experimental results indicate that the Mueller matrix microscope and the polarization parameters can facilitate the quantitative detection of breast ductal carcinoma tissues at different stages.
偏振成像已被公认为是一种用于探测复杂生物样本微观结构信息和光学特性的潜在强大技术。最近，我们通过在商用透射光显微镜上添加偏振态发生器和分析仪（PSG和PSA）报道了一种穆勒矩阵显微镜，并将其应用于鉴别伴有纤维化的人类肝脏和宫颈癌组织。在本文中，我们将穆勒矩阵显微镜应用于不同阶段人类乳腺导管癌样本的定量检测。计算并分析了不同区域和不同阶段乳腺导管组织的穆勒矩阵偏振分解和变换参数。为了进行更多的定量比较，还计算了几个广泛使用的图像纹理特征参数来表征偏振图像中的差异。实验结果表明，穆勒矩阵显微镜和偏振参数有助于对不同阶段的乳腺导管癌组织进行定量检测。
