Oh Gyungseok, Lee Kyung-Hwa, Chung Euiheon
Gwangju Institute of Science and Technology, School of Mechanical Engineering, 123 Cheomdangwagi-ro, Gwangju, 61005, South Korea.
Department of Pathology, Chonnam National University Hwasun Hospital and Medical School, Jeollanam-do, South Korea.
Biomed Opt Express. 2017 Oct 16;8(11):5013-5026. doi: 10.1364/BOE.8.005013. eCollection 2017 Nov 1.
Passive thermal imaging provides a limited differentiation between a tumor and neighboring tissue based on the temperature difference. We propose active thermodynamic contrast imaging (ATCI) with convection thermal modulators to provide more physiologically relevant parameters with high contrast such as the rate of temperature change, and thermal recovery time for tumor detection with a murine xenograft tumor model. With early stage tumors, we found the average rate of temperature change was higher in the tumor (0.22 ± 0.06 [Formula: see text]/sec) than that of neighboring tissue (0.13 ± 0.01 [Formula: see text]/sec) with heating modulation. With established tumors (volume > 100 mm), this tendency was greater. On the other hand, the thermal recovery time was shorter in tumor tissue (τ = 7.30 ± 0.59 sec) than that of neighboring tissue (τ = 11.91 ± 2.22 sec). We also found distinct thermal contrast with cooling modulation. These data suggest ATCI is a potential tumor detection modality for clinical application with its inherently label-free and physiology-based approach. Furthermore, this strategy may find applications in endoscopic tumor detection in the future.
被动热成像基于温度差异，在区分肿瘤与相邻组织方面能力有限。我们提出了一种带有对流热调制器的主动热力学对比成像（ATCI）技术，旨在利用小鼠异种移植肿瘤模型，提供更多具有高对比度的生理相关参数，如温度变化率和热恢复时间，以用于肿瘤检测。对于早期肿瘤，我们发现加热调制时肿瘤的平均温度变化率（0.22±0.06[公式：见原文]/秒）高于相邻组织（0.13±0.01[公式：见原文]/秒）。对于已形成的肿瘤（体积>100立方毫米），这种趋势更为明显。另一方面，肿瘤组织的热恢复时间（τ = 7.30±0.59秒）比相邻组织（τ = 11.91±2.22秒）短。我们还发现冷却调制时存在明显的热对比度。这些数据表明，主动热力学对比成像以其固有的无标记和基于生理学的方法，是一种具有临床应用潜力的肿瘤检测方式。此外，这种策略未来可能在内镜肿瘤检测中得到应用。
