Kanick Stephen Chad, McClatchy David M, Krishnaswamy Venkataramanan, Elliott Jonathan T, Paulsen Keith D, Pogue Brian W
Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755 USA.
Biomed Opt Express. 2014 Sep 3;5(10):3376-90. doi: 10.1364/BOE.5.003376. eCollection 2014 Oct 1.
This study investigates the hypothesis that structured light reflectance imaging with high spatial frequency patterns [Formula: see text] can be used to quantitatively map the anisotropic scattering phase function distribution [Formula: see text] in turbid media. Monte Carlo simulations were used in part to establish a semi-empirical model of demodulated reflectance ([Formula: see text]) in terms of dimensionless scattering [Formula: see text] and [Formula: see text], a metric of the first two moments of the [Formula: see text] distribution. Experiments completed in tissue-simulating phantoms showed that simultaneous analysis of [Formula: see text] spectra sampled at multiple [Formula: see text] in the frequency range [0.05-0.5] [Formula: see text] allowed accurate estimation of both [Formula: see text] in the relevant tissue range [0.4-1.8] [Formula: see text], and [Formula: see text] in the range [1.4-1.75]. Pilot measurements of a healthy volunteer exhibited [Formula: see text]-based contrast between scar tissue and surrounding normal skin, which was not as apparent in wide field diffuse imaging. These results represent the first wide-field maps to quantify sub-diffuse scattering parameters, which are sensitive to sub-microscopic tissue structures and composition, and therefore, offer potential for fast diagnostic imaging of ultrastructure on a size scale that is relevant to surgical applications.
利用具有高空间频率图案的结构光反射成像技术[公式：见原文]可用于定量绘制浑浊介质中的各向异性散射相函数分布[公式：见原文]。部分采用蒙特卡罗模拟，根据无量纲散射[公式：见原文]和[公式：见原文]（[公式：见原文]分布前两阶矩的度量）建立了解调反射率（[公式：见原文]）的半经验模型。在组织模拟体模中完成的实验表明，对频率范围为[0.05 - 0.5][公式：见原文]的多个[公式：见原文]处采样的[公式：见原文]光谱进行同步分析，能够在相关组织范围[0.4 - 1.8][公式：见原文]内准确估计[公式：见原文]，并在[1.4 - 1.75]范围内准确估计[公式：见原文]。对一名健康志愿者的初步测量显示，瘢痕组织与周围正常皮肤之间基于[公式：见原文]的对比度在宽场漫射成像中并不明显。这些结果代表了首批用于量化亚漫散射参数的宽场图谱，这些参数对亚微观组织结构和成分敏感，因此在与手术应用相关的尺寸尺度上为超微结构的快速诊断成像提供了潜力。
