School of Civil, Environmental and Architectural Engineering, Korea University, Seoul 136-714, South Korea.
School of Civil, Environmental and Architectural Engineering, Korea University, Seoul 136-714, South Korea; IT Convergence Materials R&D Group, Chungcheong Regional Division, Korea Institute of Industrial Technology, Chungnam 330-825, South Korea.
Bioresour Technol. 2015 Aug;190:543-9. doi: 10.1016/j.biortech.2015.02.066. Epub 2015 Feb 21.
To search for evidence of syntrophic acetate oxidation by cluster II Spirochaetes with hydrogenotrophic methanogens, batch reactors seeded with five different anaerobic sludge samples supplemented with acetate as the sole carbon source were operated anaerobically. The changes in abundance of the cluster II Spirochaetes, two groups of acetoclastic methanogens (Methanosaetaceae and Methanosarcinaceae), and two groups of hydrogenotrophic methanogens (Methanomicrobiales and Methanobacteriales) in the reactors were assessed using qPCR targeting the 16S rRNA genes of each group. Increase in the cluster II Spirochaetes (9.0±0.4-fold) was positively correlated with increase in hydrogenotrophic methanogens, especially Methanomicrobiales (5.6±1.0-fold), but not with acetoclastic methanogens. In addition, the activity of the cluster II Spirochaetes decreased (4.6±0.1-fold) in response to high hydrogen partial pressure, but their activity was restored after consumption of hydrogen by the hydrogenotrophic methanogens. These results strongly suggest that the cluster II Spirochaetes are involved in syntrophic acetate oxidation in anaerobic digesters.
为了寻找与氢营养型产甲烷菌共生的簇 II 螺旋体进行乙酸同化氧化的证据，用 5 种不同的厌氧污泥样品接种的批量反应器以乙酸作为唯一碳源进行厌氧操作。使用针对每组 16S rRNA 基因的 qPCR 评估了反应器中簇 II 螺旋体、两组乙酸同化产甲烷菌（甲烷杆菌科和甲烷八叠球菌科）和两组氢营养型产甲烷菌（甲烷微菌科和甲烷杆菌科）的丰度变化。簇 II 螺旋体的增加（9.0±0.4 倍）与氢营养型产甲烷菌的增加呈正相关，尤其是甲烷微菌科（5.6±1.0 倍），但与乙酸同化产甲烷菌无关。此外，簇 II 螺旋体的活性随着氢分压的升高而降低（4.6±0.1 倍），但在氢营养型产甲烷菌消耗氢气后，其活性得到恢复。这些结果强烈表明，簇 II 螺旋体参与了厌氧消化器中的乙酸同化氧化。
