Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, 5166614731, Iran.
Biol Trace Elem Res. 2018 Aug;184(2):529-535. doi: 10.1007/s12011-017-1199-4. Epub 2017 Nov 30.
Unlike the role of mesenchymal stem cells (MSCs) in regenerative medicine, their application in cell therapy can be complicated by factors such as a reduction in the proliferation potential, senescent tendency of MSCs to expand, and an age-dependent decline in their number and functions. It was shown that there is an association between exposure to electromagnetic fields (EMFs) and response to stress, cell proliferation, aging, and cell death. Furthermore, the zinc ion, as an essential trace element, was reported to be involved in the regulation of the growth and cell proliferation. In this report, ratadipose tissue-derived mesenchymal stem cells (rADSCs) were randomly divided into four groups-group I (control without any ZnSO and EMF); group II (ZnSO treatment without EMF exposed); group III (EMF exposed without ZnSO); and group ІV (EMF exposed with ZnSO)-to evaluate whether 0.14 μg/ml Zinc sulfate (ZnSO) could affect cell proliferation of rADSCs under extremely low frequency-electromagnetic field (ELF-EMF). The methyl thiazoltetrazolium (MTT) method was used to determine the cell proliferation of rADSCs following exposure to ELF-EMF in the presence of ZnSO. The immunocytochemistry method as well as flow-cytometry was used to identify the cell surface markers. Next, oil red O, alizarin red, toluidine blue, and cresyl violet staining was done to evaluate the adipogenic, osteogenic, chondrogenic, and neurogenic differentiation of rADSCs as the pluripotent capacity of rADSCs, respectively. The results showed that an exposure to ELF-EMF caused a decrease in the proliferation of rADSCs. However, the ZnSO supplementation significantly increased the cell proliferation of ELF-EMF-exposed rADSCs. In addition, in the presence of 0.14 μg/ml ZnSO, rADSCs appeared to be growing faster than the control group and ZnSO significantly decreased the doubling time of ELF-EMF-exposed rADSCs. It seems that ZnSO would be a good element to induce the cell proliferation of ELF-EMF-exposed rADSCs.
与间充质干细胞（MSCs）在再生医学中的作用不同，其在细胞治疗中的应用可能会受到多种因素的影响，例如增殖潜能降低、MSCs 扩增的衰老趋势以及数量和功能随年龄的增长而下降。有研究表明，暴露于电磁场（EMFs）与应激反应、细胞增殖、衰老和细胞死亡之间存在关联。此外，锌离子作为一种必需的微量元素，据报道参与了细胞生长和增殖的调节。在本报告中，将大鼠脂肪组织来源的间充质干细胞（rADSCs）随机分为四组-组 I（无任何 ZnSO 和 EMF 的对照）；组 II（无 EMF 暴露的 ZnSO 处理）；组 III（无 ZnSO 暴露的 EMF 暴露）；和组 IV（有 ZnSO 暴露的 EMF 暴露）-以评估 0.14μg/ml 硫酸锌（ZnSO）是否会影响 ELF-EMF 下 rADSCs 的细胞增殖。使用噻唑蓝（MTT）法测定 rADSCs 在 ELF-EMF 暴露存在 ZnSO 时的细胞增殖。使用免疫细胞化学法和流式细胞术鉴定细胞表面标志物。接下来，使用油红 O、茜素红、甲苯胺蓝和甲苯胺蓝染色分别评估 rADSCs 的成脂、成骨、软骨和成神经分化，以评估 rADSCs 的多能性。结果表明，暴露于 ELF-EMF 会导致 rADSCs 的增殖减少。然而，ZnSO 的补充显著增加了 ELF-EMF 暴露的 rADSCs 的细胞增殖。此外，在 0.14μg/ml ZnSO 的存在下，rADSCs 的生长速度似乎比对照组更快，ZnSO 显著降低了 ELF-EMF 暴露的 rADSCs 的倍增时间。似乎 ZnSO 是一种很好的元素，可以诱导 ELF-EMF 暴露的 rADSCs 的细胞增殖。
