Santina Luca Della, Ou Yvonne
Department of Ophthalmology, University of California, San Francisco, 10 Koret Way, San Francisco, CA, 94143, USA.
Methods Mol Biol. 2018;1695:161-170. doi: 10.1007/978-1-4939-7407-8_14.
Labeling of cellular structures is of fundamental importance in the investigation of diseases of the central nervous system. Biolistic labeling of retinal ganglion cells (RGCs) allows visualization of dendritic and synaptic structures of RGCs in retinal explants from animal models of experimental glaucoma. This technique sparsely labels RGCs, and, due to the stochastic nature of the particle delivery, all RGC types can be potentially observed in the labeled tissue. Quantification of dendritic and synaptic properties permits examination of the specific alterations to RGC morphology at different stages of degeneration, such as dendritic shrinkage and excitatory synapse loss.
细胞结构的标记在中枢神经系统疾病研究中至关重要。视网膜神经节细胞（RGCs）的生物弹道式标记能够使实验性青光眼动物模型视网膜外植体中的RGCs树突和突触结构可视化。该技术对RGCs进行稀疏标记，并且由于粒子递送的随机性，在标记组织中可能观察到所有RGC类型。对树突和突触特性进行定量分析有助于检查RGC形态在不同退变阶段的特定改变，如树突萎缩和兴奋性突触丧失。
