Yang Hongli, Reynaud Juan, Lockwood Howard, Williams Galen, Hardin Christy, Reyes Luke, Gardiner Stuart K, Burgoyne Claude F
Optic Nerve Head Research Laboratory, Legacy Research Institute, Devers Eye Institute, 1225 NE 2nd Ave., Portland, OR, 97232, USA.
Discoveries in Sight Research Laboratories, Legacy Research Institute, Devers Eye Institute, Portland, OR, USA.
Methods Mol Biol. 2018;1695:207-267. doi: 10.1007/978-1-4939-7407-8_17.
Accurately characterizing the 3D geometry of the optic nerve head neural and connective tissues has been the goal of a large and important body of scientific work. In the present report, we summarize our methods for the high-resolution, digital, 3D histomorphometric reconstruction of the optic nerve head tissues, including their visualization, parameterization, and quantification. In addition, we present our methods for between-eye comparisons of this anatomy, and their use to determine animal-specific and experiment-wide experimental glaucoma versus Control eye differences in the unilateral, monkey experimental glaucoma model. Finally, we demonstrate its application to finite element modeling, 3D optic nerve head reconstruction of other species, and 3D optic nerve head reconstructions using other imaging modalities.
准确描述视神经乳头神经组织和结缔组织的三维几何结构一直是大量重要科学工作的目标。在本报告中，我们总结了对视神经乳头组织进行高分辨率、数字化三维组织形态计量学重建的方法，包括其可视化、参数化和量化。此外，我们还介绍了对该解剖结构进行双眼比较的方法，以及在单侧猴子实验性青光眼模型中用于确定动物特异性和全实验性实验性青光眼与对照眼差异的方法。最后，我们展示了其在有限元建模、其他物种的三维视神经乳头重建以及使用其他成像方式进行三维视神经乳头重建中的应用。
