Lockwood Howard, Reynaud Juan, Gardiner Stuart, Grimm Jonathan, Libertiaux Vincent, Downs J Crawford, Yang Hongli, Burgoyne Claude F
Optic Nerve Head Research Laboratory, Discoveries in Sight Research Laboratories, Devers Eye Institute, Legacy Health, Portland, Oregon, United States Discoveries in Sight Research Laboratories of the Devers Eye Institute, Legacy Health, Portland, Oregon, United States.
Discoveries in Sight Research Laboratories of the Devers Eye Institute, Legacy Health, Portland, Oregon, United States.
Invest Ophthalmol Vis Sci. 2015 Feb 3;56(3):1618-37. doi: 10.1167/iovs.14-15967.
To introduce quantitative postmortem lamina cribrosa (LC) microarchitecture (LMA) assessment and characterize beam diameter (BD), pore diameter (PD), and connective tissue volume fraction (CTVF) in 21 normal monkey eyes.
Optic nerve heads (ONHs) underwent digital three-dimensional (3D) reconstruction and LC beam segmentation. Each beam and pore voxel was assigned a diameter based on the largest sphere that contained it before transformation to one of twelve 30° sectors in a common cylinder. Mean BD, PD, and CTVF within 12 central and 12 peripheral subsectors and within inner, middle, and outer LC depths were assessed for sector, subsector, and depth effects by analysis of variance using general estimating equations. Eye-specific LMA discordance (the pattern of lowest connective tissue density) was plotted for each parameter.
The ranges of mean BD, PD, and CTVF were 14.0 to 23.1 μm, 20.0 to 35.6 μm, and 0.247 to 0.638, respectively. Sector, subsector, and depth effects were significant (P < 0.01) for all parameters except subsector on CTVF. Beam diameter and CTVF were smaller and PD was larger within the superior-temporal (ST) and inferior-temporal (IT) sectors (P < 0.05). These differences were enhanced within the central versus peripheral subsectors. Beam diameter and CTVF were larger and PD was smaller (P < 0.05) within the middle LC layer. Lamina cribrosa microarchitecture discordance most commonly occurred within the ST and IT sectors, varied by eye, and generally diminished as CTVF increased.
Our data support previous characterizations of diminished connective tissue density within the ST and IT ONH regions. The clinical importance of eye-specific LMA discordance warrants further study.
介绍定量死后筛板（LC）微结构（LMA）评估，并对21只正常猴眼的梁直径（BD）、孔径（PD）和结缔组织体积分数（CTVF）进行特征描述。
对视神经乳头（ONH）进行数字三维（3D）重建和LC梁分割。在转换到一个共同圆柱体中的十二个30°扇区之一之前，根据包含每个梁和孔隙体素的最大球体为其指定一个直径。使用通用估计方程通过方差分析评估12个中央和12个外周子扇区内以及LC内、中、外深度内的平均BD、PD和CTVF的扇区、子扇区和深度效应。绘制每个参数的眼特异性LMA不一致性（最低结缔组织密度模式）。
平均BD、PD和CTVF的范围分别为14.0至23.1μm、20.0至35.6μm和0.247至0.638。除CTVF的子扇区外，所有参数的扇区、子扇区和深度效应均显著（P<0.01）。在颞上（ST）和颞下（IT）扇区内，梁直径和CTVF较小，PD较大（P<0.05）。这些差异在中央与外周子扇区内增强。在LC中层内，梁直径和CTVF较大，PD较小（P<0.05）。筛板微结构不一致最常见于ST和IT扇区内，因眼而异，并且通常随着CTVF增加而减小。
我们的数据支持先前对ST和IT ONH区域内结缔组织密度降低的特征描述。眼特异性LMA不一致的临床重要性值得进一步研究。
