Wang Lina, Wang Xin, Li Ying, Hou Yan, Sun Fengyu, Zhou Shuang, Li Chunming, Zhang Bin
Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
Institute of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
Oncotarget. 2017 Sep 27;8(54):92324-92332. doi: 10.18632/oncotarget.21289. eCollection 2017 Nov 3.
Biological requirements for tumor cell proliferation include the sustained increase of structural, energetic, signal transduction and biosynthetic precursors. Because lipids participate in membrane construction, energy storage, and cell signaling. We hypothesized that the differences in lipids between malignant carcinoma and normal controls could be reflected in the bio-fluids. A total of 100 pre-operative plasma samples were collected from 50 oral squamous cell carcinoma (OSCC), 50 normal patients and characterize by lipid profiling using ultra performance liquid chromatography/electro spray ionization mass spectrometry (UPLC-MS). The lipid profiles of the OSCC and control samples as well as the different stages were compared. Differentially expressed lipids were categorized as glycerophospholipids and sphingolipids. All glycerophospholipids were decreased, especially phosphatidylcholine and phosphoethanolamine plasmalogens, whereas sphingolipids were increased in the OSCC patients compared to the controls. We further identified 12 staging related lipids, which could be utilized to discriminate early stage patients from advanced stage patients. In the future, the differential lipids may provide biologists with additional information regarding lipid metabolism and guide clinicians in making individualized therapeutic decisions if these results are confirmed in a larger study.
肿瘤细胞增殖的生物学需求包括结构、能量、信号转导和生物合成前体物质的持续增加。由于脂质参与细胞膜构建、能量储存和细胞信号传导。我们推测恶性肿瘤与正常对照之间的脂质差异可能反映在生物流体中。从50例口腔鳞状细胞癌（OSCC）患者和50例正常患者中收集了总共100份术前血浆样本，并使用超高效液相色谱/电喷雾电离质谱（UPLC-MS）通过脂质谱分析进行表征。比较了OSCC和对照样本以及不同阶段的脂质谱。差异表达的脂质分为甘油磷脂和鞘脂。与对照组相比，所有甘油磷脂均减少，尤其是磷脂酰胆碱和磷酸乙醇胺缩醛磷脂，而OSCC患者的鞘脂增加。我们进一步鉴定出12种与分期相关的脂质，可用于区分早期患者和晚期患者。如果这些结果在更大规模的研究中得到证实，那么未来这些差异脂质可能会为生物学家提供有关脂质代谢的更多信息，并指导临床医生做出个体化的治疗决策。
