Stein A, Whitlock J P, Bina M
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5000-4. doi: 10.1073/pnas.76.10.5000.
We provide evidence that nucleosomes can assemble in vitro at physiological ionic strength (0.1-0.2 M NaCl/10 mM Tris . HCl, pH 8.0) in the absence of "assembly factors" and that poly(glutamic acid) greatly facilitates chromatin assembly under these conditions. We also show that in the presence of either poly(glutamic acid) or poly(aspartic acid), core histones assemble into octamers at physiological ionic strength. We suggest that it is a property of histones to assemble into octamers upon their interaction with macromolecules containing regions of high negative charge density, and we discuss several implications of this property.
我们提供的证据表明，在生理离子强度（0.1 - 0.2 M NaCl/10 mM Tris·HCl，pH 8.0）下，核小体能够在无“组装因子”的情况下于体外组装，并且在这些条件下聚谷氨酸极大地促进了染色质组装。我们还表明，在聚谷氨酸或聚天冬氨酸存在的情况下，核心组蛋白在生理离子强度下组装成八聚体。我们认为，组蛋白与含有高负电荷密度区域的大分子相互作用时组装成八聚体是其特性，并且我们讨论了这一特性的若干影响。
Zotero 插件
