Weintraub H, Palter K, Van Lente F
Cell. 1975 Sep;6(1):85-110. doi: 10.1016/0092-8674(75)90077-x.
In 2 M NaCl, histones H2b, H2a, H3, and H4 form a heterotypic tetrameric complex made up of one chain of each histone. This complex has been analyzed by hydrodynamic techniques. It is indistinguishable from histones in chromatin by its resistance to trypsin, pattern of reactivity with 125I. and ability to form specific crosslinked products after treatment with formaldehyde. It is proposed that this complex is responsible for protecting the small DNA fragments produced by exhausting nuclease digestion of nuclei and that on the average two of these complexes protect the larger 180-200 base pair unit produced by partial treatment of nuclei with nuclease.
在2M氯化钠溶液中，组蛋白H2b、H2a、H3和H4形成一种异型四聚体复合物，该复合物由每种组蛋白的一条链组成。已通过流体动力学技术对这种复合物进行了分析。它对胰蛋白酶的抗性、与125I的反应模式以及在用甲醛处理后形成特定交联产物的能力，使其与染色质中的组蛋白无法区分。有人提出，这种复合物负责保护通过细胞核核酸酶消化耗尽后产生的小DNA片段，并且平均而言，其中两个这样的复合物保护通过用核酸酶对细胞核进行部分处理而产生的较大的180 - 200碱基对单元。
