Landefeld T D, Campbell K L, Midgley A R
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5153-7. doi: 10.1073/pnas.76.10.5153.
Total ovarian granulosa cell proteins were labeled in vitro with [35S]methionine and analyzed by two-dimensional polyacrylamide gel electrophoresis. Of the 100-150 distinct proteins resolved by this method, 1-2% demonstrated an observable change in their synthesis during the early phases of luteinization after hormone administration. Two specific proteins first appeared as early as 30 min after treatment with human choriogonadotropin, while four additional new proteins became apparent at 3 hr. These changes in the synthesis of specific proteins occurred far in advance of the morphological changes associated with luteinization, which occur 24-72 hr later, and thus may play an important role in this process of hormonally induced cell differentiation in the rat ovary.
用[35S]甲硫氨酸对大鼠卵巢颗粒细胞的总蛋白进行体外标记，并用双向聚丙烯酰胺凝胶电泳进行分析。用该方法分离出的100 - 150种不同蛋白质中，有1 - 2%在激素给药后黄体化早期阶段其合成表现出可观察到的变化。两种特定蛋白质在用人绒毛膜促性腺激素处理后最早30分钟就出现了，而另外四种新蛋白质在3小时时变得明显。特定蛋白质合成的这些变化远早于与黄体化相关的形态学变化（后者在24 - 72小时后发生），因此可能在大鼠卵巢激素诱导的细胞分化过程中起重要作用。
