Leavitt J, Moyzis R
J Biol Chem. 1978 Apr 25;253(8):2497-500.
Proteins solubilized from the chemically transformed, highly tumorigenic Syrian hamster cell line, BP6T, and the untransformed parental embryo cells, have been analyzed by two-dimensional gel electrophoresis. Differences in seven major polypeptides have been identified in cytoplasmic and nuclear cell fractions from these two related cell types. The tumorigenic cells have lost the ability to synthesize detectable amounts of five major polypeptides which are found in untransformed cells; in addition, the tumorigenic cells synthesize two new major polypeptide species not found in the untransformed cells. Butyric acid, an agent which suppresses in vitro cellular properties frequently associated with neoplasia, induces in a reversible fashion synthesis of two of these missing polypeptide species in the tumorigenic cells. The results indicate that a change in the synthesis of less than 1% of the major polypeptide species is associated with a chemical mediated induction of the high tumorigenic state of Syrian hamster cells.
从化学转化的、具有高度致瘤性的叙利亚仓鼠细胞系BP6T以及未转化的亲代胚胎细胞中溶解出的蛋白质，已通过二维凝胶电泳进行了分析。在这两种相关细胞类型的细胞质和细胞核细胞组分中，已鉴定出七种主要多肽存在差异。致瘤细胞失去了合成未转化细胞中可检测到的五种主要多肽的能力；此外，致瘤细胞合成了两种未转化细胞中未发现的新的主要多肽种类。丁酸是一种抑制体外通常与肿瘤形成相关的细胞特性的物质，它能以可逆的方式诱导致瘤细胞中两种缺失的多肽种类的合成。结果表明，主要多肽种类中不到1%的合成变化与化学介导的叙利亚仓鼠细胞高致瘤状态的诱导有关。
