Li R, Rajan R, Wong W C V, Reid D G, Duer M J, Somovilla V J, Martinez-Saez N, Bernardes G J L, Hayward R, Shanahan C M
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Chem Commun (Camb). 2017 Dec 14;53(100):13316-13319. doi: 10.1039/c7cc06624d.
Non-enzymatic glycation of extracellular matrix with (U-C)-d-ribose-5-phosphate (R5P), enables in situ 2D ssNMR identification of many deleterious protein modifications and crosslinks, including previously unreported oxalamido and hemiaminal (CH-CH(OH)NHR) substructures. Changes in charged residue proportions and distribution may be as important as crosslinking in provoking and understanding harmful tissue changes.
细胞外基质与（U-C）-d-核糖-5-磷酸（R5P）的非酶糖基化，能够通过原位二维固体核磁共振（2D ssNMR）鉴定许多有害的蛋白质修饰和交联，包括先前未报道的草酰胺和半缩醛胺（CH-CH(OH)NHR）亚结构。带电残基比例和分布的变化在引发和理解有害组织变化方面可能与交联同样重要。
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