Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, L3 3AF, UK.
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh.
Phytother Res. 2018 Feb;32(2):348-354. doi: 10.1002/ptr.5983. Epub 2017 Nov 29.
Heritiera fomes Buch.-Ham., a mangrove plant from the Sundarbans, has adapted to a unique habitat, muddy saline water, anaerobic soil, brackish tidal activities, and high microbial competition. Endophytic fungal association protects this plant from adverse environmental conditions. This plant is used in Bangladeshi folk medicine, but it has not been extensively studied phytochemically, and there is hardly any report on investigation on endophytic fungi growing on this plant. In this study, endophytic fungi were isolated from the surface sterilized cladodes and leaves of H. fomes. The antimicrobial activities were evaluated against two Gram-positive and two Gram-negative bacteria and the fungal strain, Candida albicans. Extracts of Pestalotia sp. showed activities against all test bacterial strains, except that the ethyl acetate extract was inactive against Escherichia coli. The structures of the purified compounds, oxysporone and xylitol, were elucidated by spectroscopic means. The anti-MRSA potential of the isolated compounds were determined against various MRSA strains, that is, ATCC 25923, SA-1199B, RN4220, XU212, EMRSA-15, and EMRSA-16, with minimum inhibitory concentration values ranging from 32 to 128 μg/ml. This paper, for the first time, reports on the anti-MRSA property of oxysporone and xylitol, isolation of the endophyte Pestalotia sp. from H. fomes, and isolation of xylitol from a Pestalotia sp.
Heritiera fomes Buch.-Ham.，一种来自孙德尔本斯的红树林植物，已经适应了独特的生境，包括泥泞的盐水、缺氧土壤、咸淡水潮汐活动和高微生物竞争。内生真菌的共生关系保护了这种植物免受不利环境条件的影响。这种植物在孟加拉国的民间医学中被使用，但它在植物化学方面的研究还不够广泛，几乎没有关于在这种植物上生长的内生真菌的调查报道。在这项研究中，从表面消毒的佛焰花苞和叶子中分离出内生真菌。评估了这些内生真菌对两种革兰氏阳性菌和两种革兰氏阴性菌以及真菌菌株白色念珠菌的抗菌活性。Pestalotia sp. 的提取物对所有测试的细菌菌株都显示出活性，除了乙酸乙酯提取物对大肠杆菌没有活性。通过光谱手段阐明了纯化化合物oxysporone 和木糖醇的结构。分离得到的化合物对各种耐甲氧西林金黄色葡萄球菌 (MRSA) 菌株的抗 MRSA 潜力进行了测定，即 ATCC 25923、SA-1199B、RN4220、XU212、EMRSA-15 和 EMRSA-16，最小抑菌浓度值范围为 32 至 128 μg/ml。本文首次报道了 oxysporone 和木糖醇的抗 MRSA 特性、从 H. fomes 中分离出内生菌 Pestalotia sp.，以及从 Pestalotia sp. 中分离出木糖醇。
