Folkman J, Haudenschild C C, Zetter B R
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5217-21. doi: 10.1073/pnas.76.10.5217.
Capillary endothelial cells from rats, calves, and humans, have been carried in long-term culture. Bovine capillary endothelial cells have been cloned and maintained by serial passage for longer than 8 months. This prolonged culture was accomplished by using tumor-conditioned medium, gelatin-coated plates, and a method of enriching cells in primary culture. Cultured bovine capillary endothelial cells produce Factor VIII antigen and angiotensin-converting enzyme, but do not have Weibel-Palade bodies. Human cells do contain Weibel-Palade bodies. Capillary endothelial cells are distinguished from aortic endothelial cells by their requirement for conditioned medium. Bovine capillary endothelial cells in regular medium grow slowly with a mean doubling time of 67 hr and eventually die. In tumor-conditioned medium, these cells grow rapidly with a doubling time of 28 hr and continue to proliferate for as long as the tumor-conditioned medium is present. In contrast, bovine aortic endothelial cells grow as rapidly in regular medium as in tumor-conditioned medium. This method allows the production of pure capillary endothelial cells that may prove useful for studies of tumor angiogenesis, metastatic mechanisms, and the role of capillary endothelium in other pathologic states.
大鼠、小牛和人类的毛细血管内皮细胞已进行长期培养。牛毛细血管内皮细胞已被克隆，并通过连续传代维持了8个月以上。这种长期培养是通过使用肿瘤条件培养基、明胶包被的培养板以及一种在原代培养中富集细胞的方法来实现的。培养的牛毛细血管内皮细胞产生因子VIII抗原和血管紧张素转换酶，但没有魏贝尔-帕拉德小体。人类细胞确实含有魏贝尔-帕拉德小体。毛细血管内皮细胞与主动脉内皮细胞的区别在于它们对条件培养基的需求。在常规培养基中，牛毛细血管内皮细胞生长缓慢，平均倍增时间为67小时，最终死亡。在肿瘤条件培养基中，这些细胞生长迅速，倍增时间为28小时，只要有肿瘤条件培养基存在，就会继续增殖。相比之下，牛主动脉内皮细胞在常规培养基中和在肿瘤条件培养基中生长速度一样快。这种方法可以产生纯的毛细血管内皮细胞，这可能对肿瘤血管生成、转移机制以及毛细血管内皮在其他病理状态中的作用的研究有用。
