Zetter B R
Nature. 1980 May 1;285(5759):41-3. doi: 10.1038/285041a0.
Angiogenesis, the growth of new blood vessels, occurs normally during osteogenesis, luteinisation and the development of the embryo, and in pathological states such as chronic inflammation, certain immune reactions and neoplasia. Furthermore, solid tumours have been reported to secrete a diffusible factor which promotes the directional growth of new capillaries towards a growing tumour. Two events required for the formation of a new capillary in response to an angiogenesis factor in vivo are the migration and subsequent proliferation of capillary endothelial cells. Progress in purifying angiogenesis factors and studying their action has been hindered, however, by the lack of quantitative in vitro assays for capillary cell migration and proliferation. Recently, we have been able to isolate clonal cell lines of bovine capillary endothelial cells that can be maintained in long-term culture using tumour-conditioned growth medium. I now report a quantitative in vitro assay for endothelial cell migration based on the phagokinetic track assay of Albrecht-Buehler. The evidence presented here demonstrates that tumour-derived factors stimulate the migration of capillary endothelial cells whereas the same factors have no effect on the migration of aortic endothelial cells.
血管生成，即新血管的生长，在骨生成、黄体化和胚胎发育过程中正常发生，也发生在诸如慢性炎症、某些免疫反应和肿瘤形成等病理状态下。此外，据报道实体瘤会分泌一种可扩散因子，该因子促进新毛细血管向生长中的肿瘤定向生长。体内对血管生成因子产生反应形成新毛细血管所需的两个事件是毛细血管内皮细胞的迁移和随后的增殖。然而，由于缺乏用于毛细血管细胞迁移和增殖的定量体外测定方法，纯化血管生成因子并研究其作用的进展受到了阻碍。最近，我们已经能够分离出牛毛细血管内皮细胞的克隆细胞系，这些细胞系可以使用肿瘤条件生长培养基进行长期培养。我现在报告一种基于阿尔布雷希特 - 比勒吞噬运动轨迹测定法的内皮细胞迁移定量体外测定方法。此处提供的证据表明，肿瘤衍生因子刺激毛细血管内皮细胞的迁移，而相同因子对主动脉内皮细胞的迁移没有影响。
