Babich H, Martin-Alguacil N, Borenfreund E
Laboratory Animal Research Center, Rockefeller University, New York, NY 10021.
Toxicol Lett. 1989 Feb;45(2-3):157-64. doi: 10.1016/0378-4274(89)90005-2.
A fibroblastic cell line derived from gill tissue (designated BG/G) and an epithelioid cell line derived from fin tissue (designed BG/F) of bluegill sunfish (Lepomis macrochirus) were used as the bioindicators in toxicity experiments. The neutral red in vitro cytotoxicity assay served as the endpoint. In both cell lines the sequence of observed cytotoxicity was arsenite greater than arsenate greater than selenite greater than selenate, with each cell type exhibiting comparable ranking of midpoint toxicity (NR50) concentrations. Antagonistic interactions were noted between combinations of arsenics and seleniums. Thus, selenate and selenite (at nontoxic levels) reduced, but did not eliminate, the acute cytotoxicities of arsenate and, to a lesser extent, of arsenite.
从蓝鳃太阳鱼（Lepomis macrochirus）的鳃组织衍生而来的成纤维细胞系（命名为BG/G）和鳍组织衍生而来的上皮样细胞系（命名为BG/F）被用作毒性实验中的生物指示剂。中性红体外细胞毒性试验作为终点指标。在两种细胞系中，观察到的细胞毒性顺序为亚砷酸盐大于砷酸盐大于亚硒酸盐大于硒酸盐，每种细胞类型的半数毒性（NR50）浓度排名相当。砷和硒的组合之间存在拮抗相互作用。因此，硒酸盐和亚硒酸盐（在无毒水平）降低了砷酸盐的急性细胞毒性，在较小程度上也降低了亚砷酸盐的急性细胞毒性，但并未消除。
