Babich H, Borenfreund E
Laboratory Animal Research Center, Rockefeller University, New York, New York 10021.
Fundam Appl Toxicol. 1988 Feb;10(2):295-301. doi: 10.1016/0272-0590(88)90314-4.
The bluegill sunfish (Lepomis macrochirus) BF-2 cell line, propagated at 34 degrees C, served as target for evaluation of the acute toxicities of various classes of aquatic pollutants, using the neutral red cytotoxicity assay. For a series of chlorinated benzenes and anilines, the sequence of cytotoxicity was dependent on the degree of chlorination and on their hydrophobicity, as described by their logarithmic octanol/water partition coefficients (log P values). With increasing numbers of chlorine atoms in the ring structure or with increasing log P values, greater cytotoxicity was observed. For a series of diorganotins, the sequence of cytotoxicity was dependent on the length of the carbon chain and upon their hydrophobicity, as described by Hansch pi parameters. Thus, increasing the chain length or increasing the Hansch pi parameter resulted in greater cytotoxicity. Similar structure-activity relationships for these classes of test agents have been previously established using acute toxicity LC50 assays with aquatic species. The ability of the neutral red in vitro cytotoxicity assay, with cultured fish cells as the bioindicators, to mimic the acute toxicity data obtained from the LC50 assays suggests its utility as a tool for preliminary screening (tier I testing) of aquatic pollutants.
蓝鳃太阳鱼（Lepomis macrochirus）BF - 2细胞系在34摄氏度下传代培养，作为评估各类水生污染物急性毒性的靶标，采用中性红细胞毒性试验。对于一系列氯苯和苯胺，细胞毒性顺序取决于氯化程度及其疏水性，疏水性用它们的对数辛醇/水分配系数（log P值）来描述。随着环结构中氯原子数量增加或log P值增大，观察到细胞毒性增强。对于一系列二有机锡化合物，细胞毒性顺序取决于碳链长度及其疏水性，疏水性用汉施π参数来描述。因此，增加碳链长度或增大汉施π参数会导致细胞毒性增强。此前使用对水生物种的急性毒性LC50试验，已为这些类别的受试物建立了类似的构效关系。以培养的鱼类细胞作为生物指示剂的中性红体外细胞毒性试验，能够模拟从LC50试验获得的急性毒性数据，这表明它可用作水生污染物初步筛选（一级测试）的工具。
