Rössner P, Bencko V, Havránková H
Environ Health Perspect. 1977 Aug;19:235-7. doi: 10.1289/ehp.19-1637387.
The protective influence of arsenic against the toxic action of selenium has been tested on suspension cultures of mice fibroblasts LA 115. The growth of the cells was observed after the isolated and combined action of sodium arsenite NaAsO2 and sodium selenite Na2SeO3. The concentration range of both substances in cultivation medium MEm (USOL) was 10(-5) -10(-11)M. The growth of treated cultures was analyzed daily during 5 days of exposure. From the results obtained, growth curves of the cell cultures were constructed and analyzed. The results of every determination were evaluated in relation to the corresponding control culture. The results obtained demonstrate that decreasing concentrations of arsenic enhanced its protective effect in the range of the concentrations used. In contrast, a low protective effect of selenium against arsenic was noted in the concentrations employed. The cell cultures have proved to be very suitable for toxicological studies of the combined effects of different substances.
已在小鼠成纤维细胞LA 115悬浮培养物上测试了砷对硒毒性作用的保护影响。观察了亚砷酸钠（NaAsO₂）和亚硒酸钠（Na₂SeO₃）单独及联合作用后细胞的生长情况。培养基MEm（USOL）中这两种物质的浓度范围为10⁻⁵ - 10⁻¹¹M。在暴露的5天期间每天分析经处理培养物的生长情况。根据所得结果构建并分析了细胞培养物的生长曲线。每次测定的结果都相对于相应的对照培养物进行评估。所得结果表明，在所使用的浓度范围内，砷浓度降低增强了其保护作用。相反，在所采用的浓度下，硒对砷的保护作用较低。事实证明，细胞培养物非常适合用于不同物质联合作用的毒理学研究。
