Deutschmann S, Laib R J
Institut für Arbeitsphysiologie an der Universität Dortmund, F.R.G.
Toxicol Lett. 1989 Feb;45(2-3):175-83. doi: 10.1016/0378-4274(89)90007-6.
The effects of different exposure concentrations of butadiene on the cellular non-protein sulfhydryl (NPSH) content of liver, lung and heart tissue were investigated in B6C3F1 mice and Sprague-Dawley rats. Groups of male animals of both species were exposed for 7 h to 10, 50, 100, 250, 500, 1000 and 2000 ppm butadiene. Immediately after exposure, NPSH content of liver, lung and heart tissue was determined according to a modified Ellman procedure. A comparison of both species shows that a dose-dependent NPSH depletion can be observed in mice for all tissues examined. In rats, liver NPSH content shows a major reduction at high exposure concentrations only. In mice, depletion of NPSH content of liver, lung and heart tissue starts at exposure concentrations of about 250 ppm butadiene. A reduction in NPSH content of about 80% is observed for lung tissue at 1000 ppm and for liver and heart tissue at exposure concentrations of 2000 ppm butadiene. The data on tissue concentrations of NPSH obtained after exposure of rats and mice to butadiene reflect the quantitative differences in butadiene metabolism and in biological effectivity of reactive butadiene intermediates between both species.
在B6C3F1小鼠和Sprague-Dawley大鼠中，研究了不同暴露浓度的丁二烯对肝脏、肺和心脏组织细胞非蛋白巯基（NPSH）含量的影响。将两种物种的雄性动物分组，暴露于10、50、100、250、500、1000和2000 ppm的丁二烯中7小时。暴露后立即根据改良的埃尔曼程序测定肝脏、肺和心脏组织的NPSH含量。两种物种的比较表明，在所检查的所有组织中，小鼠均可观察到剂量依赖性的NPSH消耗。在大鼠中，仅在高暴露浓度下肝脏NPSH含量才会出现显著降低。在小鼠中，肝脏、肺和心脏组织的NPSH含量消耗在丁二烯暴露浓度约为250 ppm时开始。在1000 ppm时，肺组织的NPSH含量降低约80%，在丁二烯暴露浓度为2000 ppm时，肝脏和心脏组织的NPSH含量降低约80%。大鼠和小鼠暴露于丁二烯后获得的NPSH组织浓度数据反映了两种物种之间丁二烯代谢和活性丁二烯中间体生物有效性的定量差异。
