Department of Laboratory Medicine, University-Hospital, Padova, Italy.
Clinical Pathology Laboratory, Hospital of Adria, Adria, Italy.
Clin Chem Lab Med. 2018 Apr 25;56(5):830-837. doi: 10.1515/cclm-2017-0730.
The study endpoint was to analyze the effect of preanalytical (time, temperature, anticoagulant) and analytical (imprecision, correlation between volume and platelet concentration) variables on mean platelet volume (MPV). A further aim was to calculate in an adult population the reference intervals using the Sysmex XE-5000 analyzer. A critical evaluation was also made of the clinical utility of these parameters.
Analyses of the above values were performed in duplicate in 170 healthy adults of both sexes: (1) within 30 min from collection, and (2) after 4 h. To evaluate stability over time, the value of the platelet parameters of 20 subjects were determined, a re-analysis being performed for a period of up to 24 h on samples maintained at room temperature and 4°C using either K2-EDTA or Na-citrate as anticoagulants.
The stability over time of MPV closely depends on the anticoagulant used, storage temperature and time interval between venipuncture and analysis. An inverse, non-linear correlation between MPV and platelet count was also found.
In view of their effect on MPV and other related indices, the preanalytical and analytical variables make them, little more than experimental.
本研究的终点是分析血小板平均体积（MPV）的分析前（时间、温度、抗凝剂）和分析（不精密度、体积与血小板浓度之间的相关性）变量的影响。进一步的目的是使用希森美康 XE-5000 分析仪计算成年人群的参考区间。还对这些参数的临床实用性进行了批判性评估。
在 170 名健康的成年男女中重复进行了上述值的分析：（1）采集后 30 分钟内，（2）采集后 4 小时。为了评估随时间的稳定性，对 20 名受试者的血小板参数值进行了评估，对在室温下和 4°C 下保存的样本进行了长达 24 小时的重新分析，抗凝剂使用 K2-EDTA 或 Na-柠檬酸盐。
MPV 的随时间稳定性密切取决于所用的抗凝剂、储存温度以及采血与分析之间的时间间隔。还发现了 MPV 与血小板计数之间的反相关关系。
鉴于它们对 MPV 和其他相关指标的影响，分析前和分析变量使它们更像是实验性的。
