Kobisch M, Pennings A
Ministry of Agriculture, Veterinary Services, Ploufragan, France.
Vet Rec. 1989 Jan 21;124(3):57-61. doi: 10.1136/vr.124.3.57.
The trial involved eight large white sows obtained from a closed experimental specific pathogen free herd. Four sows (two each for an experimental vaccine and for Nobi-Vac AR) were vaccinated twice (eight weeks and two weeks before parturition) with 2 ml of vaccine administered intramuscularly. Two unvaccinated sows were used as an infected control group and two unvaccinated sows served as an uninfected control group. Forty-six piglets (28 from vaccinated sows and 18 from unvaccinated sows) were challenged by intranasal instillation of Bordetella bronchiseptica at two days of age and Pasteurella multocida type D, dermonecrotic toxin at seven days of age. Among the infected control group some piglets died and there were clinical signs of pneumonia and severe turbinate atrophy. In the vaccinated groups the results showed that immunisation of the pregnant sows had provided a good level of antibodies, which were transmitted to their offspring. There was a significant reduction in the clinical signs and no lesions were observed in the group vaccinated with the experimental vaccine and only moderate atrophy of the turbinates in the Nobi-Vac AR group. B bronchiseptica and P multocida were never recovered from the lungs of the vaccinated groups and in the nasal cavities their frequency declined with age.
该试验涉及从一个封闭的实验性无特定病原体猪群中获取的八只大型白色母猪。四只母猪（两只接种实验性疫苗，两只接种诺比-维克AR疫苗）在分娩前八周和两周时分别肌肉注射2毫升疫苗，进行两次接种。两只未接种疫苗的母猪作为感染对照组，另外两只未接种疫苗的母猪作为未感染对照组。46只仔猪（28只来自接种疫苗的母猪，18只来自未接种疫苗的母猪）在两天龄时通过滴鼻感染支气管败血波氏杆菌，在七天龄时通过滴鼻感染D型多杀性巴氏杆菌（皮肤坏死毒素）。在感染对照组中，一些仔猪死亡，出现了肺炎临床症状和严重的鼻甲萎缩。在接种疫苗的组中，结果表明，怀孕母猪的免疫产生了良好水平的抗体，并传递给了它们的后代。接种实验性疫苗的组临床症状显著减轻，未观察到病变，诺比-维克AR疫苗组仅出现中度鼻甲萎缩。支气管败血波氏杆菌和多杀性巴氏杆菌从未在接种疫苗组的肺部中检出，在鼻腔中其检出频率随年龄下降。
