Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, UK; School of Biosciences, University of Kent, Canterbury, UK.
Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, UK; School of Biosciences, University of Kent, Canterbury, UK; Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, UK.
Int J Parasitol. 2018 Mar;48(3-4):197-201. doi: 10.1016/j.ijpara.2017.10.001. Epub 2017 Nov 28.
Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children in developing areas and immunocompromised individuals. Curative therapies and vaccines are lacking, mainly due to lack of a long-term culturing system of this parasite. Here, we show that COLO-680N cells infected with two different Cryptosporidium parvum strains produce sufficient infectious oocysts to infect subsequent cultures, showing a substantial fold increase in production, depending on the experiment, over the most optimistic HCT-8 models. Oocyst identity was confirmed using a variety of microscopic- and molecular-based methods. This culturing system will accelerate research on Cryptosporidium and the development of anti-Cryptosporidium drugs.
隐孢子虫寄生虫是导致腹泻的主要原因，对发展中地区的儿童和免疫功能低下的个体构成特别威胁。目前缺乏有效的治疗方法和疫苗，主要是因为缺乏长期培养这种寄生虫的系统。在这里，我们展示了感染两种不同的微小隐孢子虫（Cryptosporidium parvum）株的 COLO-680N 细胞能够产生足够数量的感染性卵囊来感染后续的培养物，在实验中，与最乐观的 HCT-8 模型相比，产量显著增加，增加幅度取决于实验情况。通过多种基于显微镜和分子的方法确认了卵囊的身份。这种培养系统将加速对隐孢子虫的研究和抗隐孢子虫药物的开发。
