College of Horticulture, Nanjing Agricultural University, Weigang No.1, Nanjing 210095, Jiangsu, China.
Faculty of Thai Traditional Medicine, Prince of Songkla University, Hat Yai 90112, Thailand.
Food Res Int. 2017 Dec;102:681-689. doi: 10.1016/j.foodres.2017.08.034. Epub 2017 Aug 14.
Allium tuberosum (AT) is traditionally used for treating nocturnal emissions, abdominal pain, diarrhea, sexual dysfunction and asthma. This study aimed at investigating the antidiabetic and hepatoprotective activities of the butyl alcohol fraction from the methanolic extract of A. tuberosum. For the antidiabetic activity, rats were induced with diabetes by intraperitoneal injection of 150mg/kg alloxan and treated for 30days with AT extract (100, 200 and 400mg/kg). Animals were sacrificed after the study and the fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC), HDL, malondialdehyde (MDA) catalase, superoxide dismutase and glutathione levels were determined. The hepatoprotective assay, mice were pretreated for seven days with AT (100, 200 and 400mg/kg) and silymarin (100mg/kg or). Thereafter 10ml/kg of 2% v/v CCl was administered intraperitoneally on the 7th day to induce acute liver injury. Blood and liver samples were obtained and serum enzymes ALT, AST, ALP, SOD, GSH, CAT, MDA and pro-inflammatory mediators were assessed. AT significantly decrease FBG, serum TG, TC, MDA levels and significant increased HDL, SOD, GSH and CAT activities in the diabetic rats. In addition, AT significantly inhibited MDA, IL-1b, IL-6 and TNF-α levels and prevented the depletion of the antioxidant enzymes GSH, SOD and CAT activities in CCl induced liver damage. Furthermore, AT markedly reduced AST, ALT and ALP levels in the CCl treated mice groups. In conclusion, the antidiabetic and hepatoprotective effect of AT may be associated with its antioxidant and its ability to inhibit the pro-inflammatory mediators.
薤白（Allium tuberosum）传统上用于治疗夜间遗精、腹痛、腹泻、性功能障碍和哮喘。本研究旨在研究薤白甲醇提取物的丁醇部分的降血糖和保肝活性。为了研究降血糖活性，通过腹腔注射 150mg/kg 链脲佐菌素使大鼠诱导糖尿病，并使用薤白提取物（100、200 和 400mg/kg）治疗 30 天。研究结束后，处死动物，测定空腹血糖（FBG）、甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白（HDL）、丙二醛（MDA）、过氧化氢酶、超氧化物歧化酶和谷胱甘肽水平。在保肝试验中，小鼠用薤白（100、200 和 400mg/kg）和水飞蓟素（100mg/kg）预处理 7 天，然后在第 7 天腹膜内给予 10ml/kg 2%v/v CCl 以诱导急性肝损伤。采集血液和肝脏样本，测定血清酶 ALT、AST、ALP、SOD、GSH、CAT、MDA 和促炎介质。薤白显著降低糖尿病大鼠的 FBG、血清 TG、TC、MDA 水平，显著增加 HDL、SOD、GSH 和 CAT 活性。此外，薤白显著抑制 MDA、IL-1b、IL-6 和 TNF-α 水平，并防止 CCl 诱导肝损伤时抗氧化酶 GSH、SOD 和 CAT 活性的耗竭。此外，薤白显著降低 CCl 处理的小鼠组中的 AST、ALT 和 ALP 水平。总之，薤白的降血糖和保肝作用可能与其抗氧化作用及其抑制促炎介质的能力有关。
