Silva Jucélia Barbosa da, Mendes Renata de Freitas, Tomasco Vívian, Pinto Nícolas de Castro Campos, de Oliveira Luiz Gustavo, Rodrigues Matheus Nehrer, Aragão Danielle Maria de Oliveira, Aguiar Jair Adriano Kopke de, Alves Maria Silvana, Castañon Maria Christina Nogueira Marques, Ribeiro Antônia, Scio Elita
Laboratory of Bioactive Natural Products, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG 36036-900, Brazil.
Glycoconjugate Analysis Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG 36036-900, Brazil.
J Ethnopharmacol. 2017 Feb 23;198:399-406. doi: 10.1016/j.jep.2017.01.039. Epub 2017 Jan 21.
Vernonia condensata Baker (Asteraceae) is traditionally used in South American Countries as an anti-inflammatory, analgesic and hepatoprotective.
This study aimed to investigate the in vivo hepatoprotective and antioxidant, and the in vitro anti-inflammatory activities of the ethyl acetate partition (EAP) from the ethanolic extract of this medicinal plant leaves.
For the in vivo hepatoprotective activity, rats were pretreated orally for seven days with vehicle, silymarin 100mg/kg or EAP 50, 100 and 200mg/kg. Then, acetaminophen 3g/kg was also orally administrated. Animals were euthanatized 24h after the damage inducement. The levels of the serum enzymes ALT, AST and ALP were determined, as well as the triglycerides, total cholesterol and fractions. The antioxidant activity was evaluated by TBARS assay and by the measurement of glutathione reductase, superoxide dismutase and catalase activities in the rats liver tissue. The in vitro anti-inflammatory assay using Raw 264.7 cell line induced by lipopolysaccharide was conducted to verify EAP ability to inhibit pro-inflammatory cytokines.
EAP was able to inhibit all the acute biochemical alterations caused by acetaminophen overdose. EAP inhibited malondialdehyde formation, maintained the catalase and increased the glutathione reductase activities. Also, EAP decreased NO, IL-6 and TNF-α levels at concentrations from 10 to 20µg/mL. 1,5-dicaffeoylquinic acid was isolated and identified as the major compound in EAP. Apigenin, luteolin, chlorogenic acid were also identified. EAP anti-inflammatory action may be due to its antioxidant activity or its capacity to inhibit the pro-inflammatory cytokines.
These results strongly suggested that V. condensata may be useful as a possible therapy against liver damage.
凝叶斑鸠菊（菊科）在南美国家传统上被用作抗炎、镇痛和保肝药物。
本研究旨在探讨该药用植物叶乙醇提取物的乙酸乙酯部位（EAP）的体内保肝和抗氧化活性以及体外抗炎活性。
对于体内保肝活性，大鼠口服给予溶媒、100mg/kg水飞蓟宾或50、100和200mg/kg EAP预处理7天。然后，也口服给予3g/kg对乙酰氨基酚。损伤诱导24小时后对动物实施安乐死。测定血清酶ALT、AST和ALP水平，以及甘油三酯、总胆固醇和各组分。通过硫代巴比妥酸反应物（TBARS）测定法以及测量大鼠肝组织中谷胱甘肽还原酶、超氧化物歧化酶和过氧化氢酶活性来评估抗氧化活性。使用脂多糖诱导的Raw 264.7细胞系进行体外抗炎试验，以验证EAP抑制促炎细胞因子的能力。
EAP能够抑制对乙酰氨基酚过量引起的所有急性生化改变。EAP抑制丙二醛形成，维持过氧化氢酶活性并增加谷胱甘肽还原酶活性。此外，EAP在浓度为10至20μg/mL时降低了一氧化氮、白细胞介素-6和肿瘤坏死因子-α水平。分离并鉴定出1,5-二咖啡酰奎宁酸是EAP中的主要化合物。还鉴定出了芹菜素、木犀草素、绿原酸。EAP的抗炎作用可能归因于其抗氧化活性或其抑制促炎细胞因子的能力。
这些结果强烈表明凝叶斑鸠菊可能作为一种对抗肝损伤的潜在治疗方法。
