Silvero-Isidre Arturo, Morínigo-Guayuán Sergio, Meza-Ojeda Aaron, Mongelós-Cardozo Marcelo, Centurión-Wenninger Claudia, Figueredo-Thiel Susy, Sanchez Diego F, Acosta Nidia
Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay.
Departamento de Fisiopatología, Facultad de Ciencias Médicas, Universidad Nacional de Asunción, San Lorenzo, Paraguay.
Parasitol Res. 2018 Jan;117(1):189-200. doi: 10.1007/s00436-017-5693-6. Epub 2017 Dec 1.
Chagas disease is a potentially fatal disease caused by the parasite Trypanosoma cruzi, which can in some cases affect the central nervous system. The objective was to evaluate the effect of aspirin (ASA) in the behavior of mice infected with T. cruzi during the acute phase. This was an experimental study with random assignation. Twenty four BALB/c mice were divided into four groups of six animals each as follows: only ASA (OA), ASA before infection (BI), ASA after infection (AI) and only infection (OI). The strain used for infection was M/HOM/Bra/53/Y. An ASA dose of 100 mg/kg per day was administered 72 h before infection to BI group and the same dose 48 h after infection to AI group. Mice behavior in the open field test, mortality, and brain histopathology was evaluated. Data were analyzed using ANOVA, chi square test, and Kaplan-Meier with long-rank for survival analysis. In the open field test, the OA group has similar results with the BI group, in the variables of immobility and escape. Also, the OA group displayed significantly higher rates of micturition (p < 0.001) and defecation (p < 0.001) compared to infected groups. Mortality was higher in BI group (p = 0.02). The presence of T. cruzi amastigotes were higher in brain tissues of the AI and OI groups (p = 0.008). In conclusion, the administration of ASA before infection seemed to prevent behavioral changes induced by the acute infection, but it led to accelerated mortality. The study highlighted the potential importance of the pathways inhibited by ASA in the early hours of acute infection with T. cruzi.
恰加斯病是一种由克氏锥虫寄生虫引起的潜在致命疾病，在某些情况下会影响中枢神经系统。目的是评估阿司匹林（ASA）对急性期感染克氏锥虫小鼠行为的影响。这是一项随机分配的实验研究。将24只BALB/c小鼠分为四组，每组6只动物，如下所示：仅使用ASA（OA）组、感染前使用ASA（BI）组、感染后使用ASA（AI）组和仅感染（OI）组。用于感染的菌株是M/HOM/Bra/53/Y。BI组在感染前72小时给予每天100mg/kg的ASA剂量，AI组在感染后48小时给予相同剂量。评估小鼠在旷场试验中的行为、死亡率和脑组织病理学。使用方差分析、卡方检验和用于生存分析的带有对数秩的Kaplan-Meier法分析数据。在旷场试验中，OA组在不动和逃避变量方面与BI组结果相似。此外，与感染组相比，OA组排尿（p<0.001）和排便（p<0.001）的发生率显著更高。BI组的死亡率更高（p=0.02）。AI组和OI组脑组织中克氏锥虫无鞭毛体的存在更高（p=0.008）。总之，感染前给予ASA似乎可以预防急性感染引起的行为变化，但会导致死亡率加速上升。该研究强调了ASA在克氏锥虫急性感染早期所抑制的途径的潜在重要性。
