Laboratory of Parasitology, Department of Bacteria, Parasites & Fungi, Infectious Disease Preparedness, Statens Serum Institut, Artillerivej 5, DK-2300, Copenhagen S, Denmark.
University of Helsinki, Helsinki, Finland.
Eur J Clin Microbiol Infect Dis. 2018 Mar;37(3):579-586. doi: 10.1007/s10096-017-3152-z. Epub 2017 Dec 3.
Genetic variation within Toxoplasma gondii can have both clinical and epidemiological significance, while the genotypes circulating in many parts of the world, including the Nordic country Denmark, are still unknown. We genetically characterized T. gondii strains that had been detected in human clinical samples in Denmark in 2011-2016. Samples that had tested positive for T. gondii DNA and had a quantification cycle value <33 were included in this study and subjected to direct genetic characterization of T. gondii based on length-polymorphism of 15 microsatellite markers. A total of 23 DNA samples from 22 individual patients were analyzed. The results were consistent with genotype II with 15/15 markers amplified from seven samples from the central nervous system (CNS) including two samples from one patient, four ocular samples, and one unspecified sample; with genotype III with 15/15 markers amplified from two ocular samples; with genotype Africa 1 with 15/15 markers amplified from one amniotic fluid sample and from one CNS-sample; with atypical genotype with 15/15 markers amplified from one CNS-sample and with 11/15 markers amplified from one CNS-sample; and with HG12-like genotype with 9/15 markers amplified from one CNS-sample. Genotype II, which is endemic in Europe, was predominant, but more than a third of the successfully genotyped strains were non-type-II. The possibility that clinical toxoplasmosis is caused by a strain that is not considered endemic to the region is definitely not negligible.
刚地弓形虫内的遗传变异可能具有临床和流行病学意义，而包括北欧国家丹麦在内的世界许多地区流行的基因型仍不清楚。我们对 2011 年至 2016 年在丹麦人类临床样本中检测到的刚地弓形虫株进行了基因特征分析。本研究纳入了刚地弓形虫 DNA 检测呈阳性且定量循环值<33 的样本，并对其进行了基于 15 个微卫星标记长度多态性的直接基因特征分析。共分析了来自 22 名个体患者的 23 个 DNA 样本。结果与基因型 II 一致，7 个来自中枢神经系统（CNS）的样本中扩增了 15/15 个标记，其中包括来自一名患者的两个样本、4 个眼部样本和 1 个未指定样本；2 个眼部样本扩增了 15/15 个标记，为基因型 III；1 个羊水样本和 1 个 CNS 样本扩增了 15/15 个标记，为基因型 Africa 1；1 个 CNS 样本扩增了 15/15 个标记和 1 个 CNS 样本扩增了 11/15 个标记，为非典型基因型；1 个 CNS 样本扩增了 9/15 个标记，为 HG12 样基因型。在欧洲流行的基因型 II 占主导地位，但超过三分之一的成功基因分型的菌株并非 II 型。临床弓形体病由一种被认为不属于该地区的菌株引起的可能性肯定不容忽视。
