Heming T A
Aquatic Toxicology Section, Alberta Environmental Centre, Vegreville, Canada.
Am J Vet Res. 1989 Jan;50(1):93-7.
Effects of blood sample collection and measurement techniques were assessed for blood gas tensions, acid-base status, and hematologic and plasma biochemical values of rainbow trout. Blood samples were collected via intraaortic cannulae from immersed, unrestrained fish and from emersed, restrained fish. The samples were analyzed at either fish body temperature (10 to 14 C) or clinical blood analyzer temperature (37 C); results obtained at 37 C were back-adjusted to fish body temperature, using standard mammalian temperature-correction factors. Fish emersion and handling for 30 seconds significantly (P less than 0.05) altered blood PCO2, acid-base status, and hematologic and plasma biochemical values. The results were consistent with respiratory acidosis and hemoconcentration. The use of mammalian temperature-correction factors for determination of fish blood gas tensions and acid-base status yielded values that were significantly (P less than 0.05) different from those measured directly at fish body temperature.
对虹鳟鱼的血气张力、酸碱状态以及血液学和血浆生化值评估了血样采集和测量技术的影响。通过主动脉插管从浸在水中、未受限制的鱼以及离水、受限制的鱼采集血样。样品在鱼体温度（10至14摄氏度）或临床血液分析仪温度（37摄氏度）下进行分析；在37摄氏度下获得的结果使用标准哺乳动物温度校正因子回调到鱼体温度。鱼离水并处理30秒会显著（P小于0.05）改变血液PCO2、酸碱状态以及血液学和血浆生化值。结果与呼吸性酸中毒和血液浓缩一致。使用哺乳动物温度校正因子来测定鱼的血气张力和酸碱状态所得到的值与在鱼体温度下直接测量的值显著（P小于0.05）不同。
