从蚕豆中分离出的摩洛哥根瘤菌的表型和遗传多样性及可能参与其耐渗性的基因研究。
Phenotypic and genetic diversity of Moroccan rhizobia isolated from Vicia faba and study of genes that are likely to be involved in their osmotolerance.
机构信息
Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakech, Morocco; Technische Universität Dresden, Institut für Genetik, Helmholtzstr. 10, D-01069 Dresden, Germany.
Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakech, Morocco; Department of Biology, Faculty of Science and Techniques, BP. 509, 52000 Boutalamine, Errachidia, Morocco.
出版信息
Syst Appl Microbiol. 2018 Jan;41(1):51-61. doi: 10.1016/j.syapm.2017.09.003. Epub 2017 Nov 3.
Rhizobia are symbiotic nitrogen-fixing bacteria in root nodules of legumes. In Morocco, faba bean (Vicia faba L.), which is the main legume crop cultivated in the country, is often grown in marginal soils of arid and semi-arid regions. This study examines the phenotypic diversity of rhizobia nodulating V. faba isolated from different regions in Morocco for tolerance to some abiotic stresses. A total of 106 rhizobia strains isolated from nodules were identified at the species level by analysing 16S rDNA. Additionally, for selected strains recA, otsA, kup and nodA fragments were sequenced. 102 isolates are likely to belong to Rhizobium leguminosarum or R. laguerreae and 4 isolates to Ensifer meliloti. All strains tolerating salt concentrations of 428 or 342mM NaCl as well as 127 or 99mM Na2SO4 were highly resistant to alkaline conditions (pH 10) and high temperature (44°C). Three strains: RhOF4 and RhOF53 (both are salt-tolerant) and RhOF6 (salt-sensitive) were selected to compare the influence of different levels of salt stress induced by NaCl on growth and on trehalose and potassium accumulation. We find a direct correlation between the trehalose contents of the rhizobial strains and their osmotolerance.
根瘤菌是与豆科植物根瘤共生的固氮细菌。在摩洛哥，作为该国主要的豆类作物之一的蚕豆（Vicia faba L.）常种植在干旱和半干旱地区的边缘土壤上。本研究考察了从摩洛哥不同地区分离的蚕豆根瘤共生根瘤菌对一些非生物胁迫的耐受性的表型多样性。通过分析 16S rDNA，对从根瘤中分离的 106 株根瘤菌菌株进行了种水平鉴定。此外，还对选定的菌株 recA、otsA、kup 和 nodA 片段进行了测序。102 株分离株可能属于根瘤菌属（Rhizobium leguminosarum 或 R. laguerreae），4 株属于慢生根瘤菌属（Ensifer meliloti）。所有耐受 428 或 342mM NaCl 以及 127 或 99mM Na2SO4 盐浓度的菌株均对碱性条件（pH 10）和高温（44°C）高度耐受。选择了 3 株菌株：RhOF4 和 RhOF53（均耐盐）和 RhOF6（盐敏感），以比较由 NaCl 引起的不同盐胁迫水平对生长和海藻糖及钾积累的影响。我们发现根瘤菌菌株中海藻糖含量与其耐渗性之间存在直接相关性。
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