Institute for Bioprocess and Analytical Measurement Technology e.V. Rosenhof, 37308, Heilbad Heiligenstadt, Germany.
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), Am Mühlenberg 13, 14476, Potsdam, Germany.
Anal Bioanal Chem. 2018 Feb;410(5):1453-1462. doi: 10.1007/s00216-017-0786-8. Epub 2017 Dec 3.
An aptamer-based biosensor was developed for the detection of doxorubicin using electrochemical impedance spectroscopy. Doxorubicin and its 14-dehydroxylated version daunorubicin are anthracyclines often used in cancer treatment. Due to their mutagenic and cardiotoxic effects, detection in groundwater is desirable. We developed a biosensor using the daunorubicin-binding aptamer as biological recognition element. The aptamer was successfully co-immobilized with mercaptohexanol on gold and a density of 1.310 ± 2.410 aptamer molecules per cm was achieved. The binding of doxorubicin to the immobilized aptamer was detected by electrochemical impedance spectroscopy. The principle is based on the inhibition of electron transfer between electrode and ferro-/ferricyanide in solution caused by the binding of doxorubicin to the immobilized aptamer. A linear relationship between the charge transfer resistance (R ) and the doxorubicin concentration was obtained over the range of 31 nM to 125 nM doxorubicin, with an apparent binding constant of 64 nM and a detection limit of 28 nM. With the advantages of high sensitivity, selectivity, and simple sensor construction, this method shows a high potential of impedimetric aptasensors in environmental monitoring. Graphical abstract Measurement chamber and immobilization principle for the detection of doxorubicin by electrochemical impedance spectroscopy.
基于适体的生物传感器用于电化学阻抗谱法检测阿霉素。阿霉素及其 14-去羟基衍生物柔红霉素是常用于癌症治疗的蒽环类抗生素。由于它们具有致突变和心脏毒性作用，因此希望在地下水检测到它们。我们使用柔红霉素结合适体作为生物识别元件开发了一种生物传感器。适体成功地与巯基己醇共固定在金上，并且达到了 1.310 ± 2.410 个/平方厘米的适体分子密度。通过电化学阻抗谱法检测到阿霉素与固定化适体的结合。其原理基于阿霉素与固定化适体结合导致溶液中电极和亚铁/铁氰化物之间电子转移的抑制。在 31 nM 至 125 nM 阿霉素范围内，电荷转移电阻（R ）与阿霉素浓度之间呈线性关系，表观结合常数为 64 nM，检测限为 28 nM。该方法具有灵敏度高、选择性好、传感器结构简单等优点，在环境监测中显示出电化学适体传感器具有很高的潜力。
