State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai, 200436, China.
School of Medicine, Shanghai University, Shanghai, 200444, People's Republic of China.
Mikrochim Acta. 2024 Feb 23;191(3):154. doi: 10.1007/s00604-024-06226-2.
Therapeutic drug monitoring of doxorubicin (DOX) is important to study pharmacokinetics in patients undergoing chemotherapy for reduction of side effects and improve patient survival by rationally controlling the dose of DOX. A fast and ultra-sensitive surface plasmon resonance (SPR) detector without sample pre-handling was developed for DOX monitoring. First, the two-dimensional metal-organic framework was modified on the Au film to enhance SPR, and then, the supramolecular probes with tunable cavity structure were self-assembled at the sensing interface for direct detection of DOX through specific host-guest interactions with a low detection limit of 60.24 pM. The precise monitoring of DOX in serum proved the possibility of clinical application with recoveries in the range 102.86-109.47%. The mechanisms of host-guest interactions between supramolecular and small-molecule drugs were explored in depth through first-principles calculations combined with SPR experiments. The study paves the way for designing facile and sensitive detectors and provides theoretical support and a new methodology for the specific detection of small molecules through calixarene cavity modulation.
阿霉素（DOX）的治疗药物监测对于研究接受化疗的患者的药代动力学非常重要，通过合理控制 DOX 剂量，可以减少副作用并提高患者生存率。本文开发了一种无需样品预处理的快速超灵敏表面等离子体共振（SPR）检测器，用于 DOX 监测。首先，在 Au 膜上修饰二维金属有机骨架以增强 SPR，然后，通过具有可调腔结构的超分子探针在传感界面上自组装，通过特定的主客体相互作用进行 DOX 的直接检测，检测限低至 60.24 pM。通过精确监测血清中的 DOX，证明了该方法具有临床应用的可能性，回收率在 102.86-109.47%范围内。通过结合 SPR 实验的第一性原理计算，深入探讨了超分子与小分子药物之间的主体-客体相互作用机制。该研究为设计简便灵敏的检测器铺平了道路，并通过杯芳烃腔调制对小分子的特异性检测提供了理论支持和新方法。
