从绿藻石莼中提取的硫酸岩藻聚糖的结构、在水溶液中的构象及抗菌活性
Structure, conformation in aqueous solution and antimicrobial activity of ulvan extracted from green seaweed Ulva reticulata.
作者信息
Tran Thi Thanh Van, Truong Hai Bang, Tran Nguyen Ha Vy, Quach Thi Minh Thu, Nguyen Thi Nu, Bui Minh Ly, Yuguchi Yoshiaki, Thanh Thi Thu Thuy
机构信息
a Nha Trang Institute of Technology Research and Application , Vietnam Academy of Science and Technology , Nha Trang , Vietnam.
b Institute of Chemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam.
出版信息
Nat Prod Res. 2018 Oct;32(19):2291-2296. doi: 10.1080/14786419.2017.1408098. Epub 2017 Dec 4.
The aim of this study is to elucidate the structure and investigate the antimicrobial activity of an ulvan obtained by water extraction from green seaweed Ulva reticulata collected at Nha Trang sea of Vietnam by using IR, NMR, SEC-MALLS and SAXS methods. The ulvan is composed of rhamnose, galactose, xylose, manose and glucose (mole ratio Rha: Gal: Xyl: Man: Glu = 1:0.12:0.1:0.06:0.03), uronic acid (22.5%) and sulphate groups (17.6%). Chemically structural determination showed that the ulvan mainly composed of disaccharide [→4)β-D-GlcA(1→4)α-L-Rha3S-(1→]. The results from SAXS indicated that ulvan under study has a rod-like bulky chain conformation. Ulvan from U. reticulata showed high antimicrobial activity, with inhibition zone diameter of 20 mm against Enterobacter cloace and 18 mm against Escherichia coli.
本研究的目的是通过红外光谱（IR）、核磁共振（NMR）、尺寸排阻色谱-多角度激光光散射联用（SEC-MALLS）和小角X射线散射（SAXS）方法，阐明从越南芽庄海域采集的绿藻石莼水提取物中获得的一种岩藻聚糖硫酸酯的结构，并研究其抗菌活性。该岩藻聚糖硫酸酯由鼠李糖、半乳糖、木糖、甘露糖和葡萄糖（摩尔比Rha:Gal:Xyl:Man:Glu = 1:0.12:0.1:0.06:0.03）、糖醛酸（22.5%）和硫酸基团（17.6%）组成。化学结构测定表明，该岩藻聚糖硫酸酯主要由二糖[→4)β-D-GlcA(1→4)α-L-Rha3S-(1→]组成。SAXS结果表明，所研究的岩藻聚糖硫酸酯具有棒状的大分子链构象。石莼中的岩藻聚糖硫酸酯表现出较高的抗菌活性，对阴沟肠杆菌的抑菌圈直径为20毫米，对大肠杆菌的抑菌圈直径为18毫米。
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